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Key considerations before commencing cell line development

Drug Target Review

This selectable marker enables the identification of cells which have taken up the GOIs and aids the initial selection of recombinant protein expressing cell lines. They work in the following way: the genes in an expression vector are flanked by inverted terminal repeat sequences (ITRs) to which a specific transposase enzyme can bind.

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The Making of a Gene Circuit

Codon

Building a Circuit Much like electronic circuits, which use wires and transistors to create or process signals, a synthetic gene circuit is a network of biological parts — DNA, RNA, and proteins — that process inputs and generate outputs. Elowitz inserted his assembled DNA into E. 3 Subscribe to Asimov Press.

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