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This selectable marker enables the identification of cells which have taken up the GOIs and aids the initial selection of recombinant proteinexpressing cell lines. They work in the following way: the genes in an expression vector are flanked by inverted terminal repeat sequences (ITRs) to which a specific transposase enzyme can bind.
Building a Circuit Much like electronic circuits, which use wires and transistors to create or process signals, a synthetic gene circuit is a network of biological parts — DNA, RNA, and proteins — that process inputs and generate outputs. Elowitz inserted his assembled DNA into E. 3 Subscribe to Asimov Press.
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