Broad Institute

JANUARY 14, 2025

Not long after the development of CRISPR-Cas9 gene editing in 2013, Vallabh and Minikel began thinking about whether CRISPR could be used to disrupt the gene encoding the prion protein. The wife-and-husband team started a lab at the Broad with a singular focus: preventing and treating prion disease within their lifetime.

Disease 144

Disease DNA Protein Production Treatment 144

Drug Target Review

NOVEMBER 1, 2023

We support the drug discovery projects of the CCDD with assay development and screening, biophysics, structural biology and recombinant protein production. With all these diverse methods we characterise protein-ligand interactions in many ways. 2013) 56, 2059-2073. The figure was generated with CCP4MG (McNicholas et al.

Fragment Screening 96

Fragment Screening Biochemical Assays Small Molecule Biophysical Assays 96

PLOS: DNA Science

JUNE 13, 2024

The most compelling evidence for the origin lies in the RNA genome sequence similarities between RaTG13 and SARS-CoV-2 – specifically, a part that encodes the “furin binding site” of the spike protein with which the virus adheres to host cells. Coincidence? I’ve never thought so. RaTG13 shares about 96.1%

Virus 52

Virus RNA DNA Science 52

BMG Labtech

FEBRUARY 24, 2025

8 A second sandwich ELISA with absorbance detection allowed measurement of the concentrations of the S100 calcium-binding protein (S100B). Advanced oxidation protein products were also determined (Fig. 2013 Mar 19;110(12):E1102-11. Epub 2013 Mar 4. Nitration of Hsp90 induces cell death. Proc Natl Acad Sci U S A.

Small Molecule 52

Small Molecule Disease Licensing Licensing 52

Codon

AUGUST 10, 2023

PNAS (2013). Link The PURE system for the cell-free synthesis of membrane proteins , by Kuruma Y. & Link An integrated cell-free metabolic platform for protein production and synthetic biology , by Jewett M.C. Annual Review of Chemical and Biomolecular Engineering (2013). & Charpentier E. & Ueda T.

DNA 97

DNA Cell Biology Molecular Biology Engineering 97

Codon

AUGUST 10, 2023

Nature Methods (2013). PNAS (2013). Link An integrated cell-free metabolic platform for protein production and synthetic biology , by Jewett M.C. Molecular Systems Biology (2013). Annual Review of Chemical and Biomolecular Engineering (2013). . & Reinberg D. Cell (2002). Nature Genetics (2002).

DNA 52

DNA Cell Biology Engineering Molecular Biology 52