article thumbnail

A covalent compound selectively inhibits RNA demethylase ALKBH5 rather than FTO

Covalent Modifiers

2024 , DOI: 10.1039/D3CB00230F N6-Methyladenosine (m6A) is the most prevalent mRNA modification and is required for gene regulation in eukaryotes. Moreover, TD19 displays good anticancer efficacy in acute myeloid leukemia and glioblastoma multiforme cell lines.

RNA 157
article thumbnail

Inside The Altascientist Issue 41: Immunomodulation Assessments for Clinical Trials–Sophisticated Bioanalytical Approaches to Support Complex Modalities

Alta Sciences

For example, ELISA and multiplexed assays are used for quantifying proteins or cytokines, ELISpot is ideal for detecting antigen-specific T-cell responses, flow cytometry helps assess cell populations and functions, and cell-based assays are essential for evaluating the functional activity of immune cells.

Insiders

Sign Up for our Newsletter

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

article thumbnail

An industry leader’s perspective on the complexity of scientific data

Drug Target Review

Data-driven discovery As with many early-stage biotech companies, Five Alarm Bio is highly data-driven, producing vast amounts of experimental data through cell-based assays and phenotypic screening. cited 2024 Oct 17]. Reference 1 Five Alarm Bio.

article thumbnail

Chemical con artists foil drug discovery

Molecular Design

The effects of curcumin, EGCG (epigallocatechin gallate), genistein and resveratrol on four membrane proteins were reported in Ref9 and these effects would raise doubts about activity for any of these compounds (or their close structural analogs) that had been observed in a cell-based assay.

Drugs 96
article thumbnail

AI At The Frontier: Empowering Early Career Professionals In Drug Discovery

Elrig

This involved designing and implementing biological strategies, encompassing target validation, hit identification, establishing assays, and collaborating on structural work. Joining Isomorphic Labs in October 2024, I’m thrilled to be part of their mission to revolutionize DD through AI/ML.

article thumbnail

Leadeth me unto Truth and delivereth me from those who have already found it

Molecular Design

With apologies to Manfred Eigen (1927 - 2019) I've just returned to Cheshire from the Caribbean and, to kick off blogging from 2024 I'll share a photo of the orchids at Berwick-on-Sea on the north coast of Trinidad. We have to make assumptions in these situations and we also need to check that these assumptions are reasonable.

Drugs 60
article thumbnail

Article FDA Thank You Draft guidance on potency assays for CGT products garners extensive stakeholder input

Agency IQ

Draft guidance on potency assays for CGT products garners extensive stakeholder input Late last year, the FDA published a draft update to its 2011 guidance on potency assays for cell and gene therapy products, unveiling a major shift in approach to the issue.