Chemical Biology and Drug Design

MARCH 27, 2024

OVCAR-3 and SKOV-3 cells were treated with diosgenin, cellular viability was assessed by MTT assay and apoptosis was measured by ELISA and evaluated the protein expression levels of apoptotic markers through western blotting. The protein expression levels of main components of PI3K signaling were evaluated via western blotting.

Protein Expression 100

Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 5, 2024

Curcumin modulates the gene and protein expression levels of ferroptosis mediators via JNK signaling. In addition, curcumin modulated the mRNA and protein expression levels of ferroptosis-related proteins including ACSL4, GPx4 and FTH1 and suppression of JNK signaling.

Protein Expression 100

Protein Expression Regulations Therapies Treatment 100

Chemical Biology and Drug Design

JUNE 21, 2023

The results showed that hsa-miR-503-5p showed high expression, while its target gene CTDSPL presented decreased expression in LUAD. Hsa-miR-503-5p also had high expression in cisplatin-resistant LUAD cells. Binding relationship between the two genes was verified by dual-luciferase reporter assay.

Regulations 100

Regulations Protein Expression Bioinformatics Drugs 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Inflammatory factors in the serum were measured using ELISA assays, immunohistochemistry and qRT-PCR were performed to determine macrophage numbers and the expression of iNOS, CD86, Arg-1, and Mrc1 in myocardial tissue. The protein expression levels of TLR4, Myd88, and NF-κB in myocardial tissue were assessed through western blot analysis.

Biochemical Assays 100

Biochemical Assays Protein Expression Drugs Research 100

Chemical Biology and Drug Design

DECEMBER 20, 2023

The protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), matrix metalloproteinase (MMP)-2, and MMP-9 were detected by western blotting. Besides, the decreased Nrf2 expression reduced the protein expression levels of MMP-2 and MMP-9 ( p  < .05).

Protein Expression 100

Protein Expression Treatment Drugs Research 100

Chemical Biology and Drug Design

SEPTEMBER 26, 2024

Western blot and molecular docking indicated that compound 22 may exert antioxidant activity by activating Nrf2 protein expression. This might be due to the introduction of 2, 5-difluorobenzene sulfonate group in PF, which helps in scavenging free radicals under oxidative stress.

Protein Expression 100

Protein Expression Pharmaceuticals Treatment Disease 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

Moreover, mechanism research illustrates these natural compounds treat influenza A virus through key targets TLR4, TNF, and IL6 (high fever, acute respiratory distress syndrome), MAPK1, and MAPK3 (MAPK signaling pathway, viral RNP export, and viral protein expression), IL1B (NOD-like receptor signaling pathway, suppressed maturation of pro-IL-1β and (..)

Virus 100

Virus Protein Expression Vaccine Research 100

Chemical Biology and Drug Design

FEBRUARY 19, 2024

SIRT1 expression was enhanced by Icariin, and its knockdown suppressed Icariin-induced BMSC osteogenic differentiation. Moreover, deubiquitinating enzyme USP47 could stabilize SIRT1 protein expression.

Regulations 100

Regulations Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 5, 2024

The results showed that CA-COS inhibited nitric oxide (NO) production and downregulated the gene expression of nitric oxide synthase (iNOS), and cytokines such as tumor necrosis factor-alpha (TNF-α), IL-1β, and IL-6 without cytotoxic effect.

Protein Expression 100

Protein Expression Therapies Disease Production 100

Chemical Biology and Drug Design

AUGUST 23, 2023

We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. The protein expressions were detected via western blot. Ligustrazine at doses of 100 and 1000 μg/mL was administrated in Kupffer cells isolated from THS rats.

Protein Expression 100

Protein Expression Protein Production Treatment Production 100

Chemical Biology and Drug Design

AUGUST 21, 2023

Further, the study detected the effect of SA on cell apoptosis, lipid peroxidation, Fe 2+ level, and ferroptosis-related proteins expression. Finally, the effect of HMGB1 expression on SA in H/R stimulation was studied.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 2, 2023

Linagliptin administered to the type 1 diabetic mouse heart significantly reduced the expression levels of the total and cleaved forms of ATF6, ATF4, and p-JNK, caspase 3. According to ELISA findings, TUDCA was more effective in reducing NOX 1 and MDA levels than linagliptin.

Protein Expression 100

Protein Expression Therapies Treatment Research 100

Chemical Biology and Drug Design

APRIL 15, 2023

The mRNA and protein expression levels of p53, p-glycoprotein (P-gp), ATM, ATR, CHK1, and CHK2 were assessed through qRT-PCR and western blotting. OECM-1 and OECM-1/PTX were transfected with miR-34 mimic and inhibitor. Cellular proliferation and apoptosis were evaluated through MTT assay and flow cytometry, respectively.

DNA 100

DNA Protein Expression Research 100

Chemical Biology and Drug Design

FEBRUARY 15, 2024

RT-qPCR and western blotting were used to test the mRNA and protein expression levels of IL-17 and retinoid-related orphan receptor-γt (RORγt). PGE2 was highly expressed in the DE mouse model. The mRNA and protein levels of IL-17 and the key Th17 transcription factor RORγt were increased in tissues of the DE mice.

Disease 100

Disease Protein Expression Regulations Treatment 100

Chemical Biology and Drug Design

SEPTEMBER 21, 2023

Colonic pathological changes were analyzed by hematoxylin–eosin staining, and inflammatory factor expressions in serum were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and western blot were performed to quantify ferroptosis-related protein expressions.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 20, 2023

Hederagenin attenuated HG-induced increase in mRNA and protein expression of NLRP3, ASC, and IL-1β. The secretion levels of fibrosis-related biomarkers were analyzed by ELISA. Results showed that hederagenin reduced HG-induced proliferation increase in HRMCs and HRPTEpiCs. IV, PAI-1, and TGF-β1.

Bioinformatics 100

Bioinformatics Protein Expression Research 100

Chemical Biology and Drug Design

JUNE 8, 2023

In addition, SAS treatment caused a significant decrease in the mRNA and protein expression of xCT and GPX4, and a significant increase in ACSL4 expression in TE-1 cells treated with SAS. Flow cytometry results showed that the ferroptosis level was significantly increased after SAS treatment.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 9, 2023

A label-free proteomics technology was employed to investigate alterations in protein expression in LoVo cells treated with plumbagin. Cell cycle analysis and cell apoptosis analysis were conducted to break down the anticancer impact of plumbagin on LoVo cells.

Bioinformatics 100

Bioinformatics Protein Expression Treatment Research 100

Chemical Biology and Drug Design

SEPTEMBER 25, 2023

Also, Vinp downregulated α-Syn protein expression and MDA level, while upregulated SOD activity in the striatum of PD rats. Vinp treatment increased the horizontal movement frequency and number of squares crossed, reduced the contact time, and rotation frequency in PD rats.

Disease 100

Disease Protein Expression Treatment Research 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

The mRNA or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real-time polymerase chain reaction or western blot.

Regulations 100

Regulations Protein Expression Treatment Research 100

Chemical Biology and Drug Design

DECEMBER 8, 2023

BoNT/A (1 nM) attenuated LPS/ATP-stimulated inhibition of viability and CAMP expression and upregulation of inflammatory mediators, pyroptosis-related proteins, and ELANE expression in rat DRG neurons, which was counteracted by CAMP silencing.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Elisa's result showed that CQQNC can significantly decrease the expression levels of cPLA2, sPLA2, PGE2, cAMP, and 15-PGDH after stimulating IL-1β to bEnd.3 3 cells in a dose-dependent manner ( p  < .01, 01, p  < .001, 001, p  < .0001). 3 ( p  < .0001, 0001, p  < .001, 001, p  < .01).

Treatment 100

Treatment Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 4, 2024

The expression of miR-181a-3p was inhibited; however, SHQ1 expression was increased by β-sitosterol treatment of A549/anlotinib cells. The inhibition of SHQ1, ATF6, and GRP78 protein expression by β-sitosterol in A549/anlotinib cells was rescued by increased miR-181a-3p.

Protein Expression 100

Protein Expression Regulations Treatment Drugs 100

Chemical Biology and Drug Design

DECEMBER 6, 2023

In the analysis of network pharmacology, 269 pharmacological targets of SP, 449 pharmacological targets of FC, and 2569 targets of HS-related diseases were screened from the databases.

Molecular Biology 100

Molecular Biology Protein Expression Treatment Regulations 100

Chemical Biology and Drug Design

DECEMBER 15, 2023

μM), significantly and dose-dependently induced apoptosis of SCC-12 and SK-MEL-28 cells, as evidenced by the suppression of Bcl-2 and upregulation of Bax, cleaved caspase-3, caspase-9, and PARP protein expression levels. The most active compounds 11 (A431: IC 50  = 5.0 μM, μM, SCC-12: IC 50  = 2.9 μM, μM, SKMEL-28: IC 50  = 4.9 μM,

Small Molecule 100

Small Molecule Protein Expression Laboratories Research 100

Drug Target Review

MARCH 15, 2024

Efforts have focused on Chinese hamster ovary (CHO) cell line improvements since it is a commonly used host for the expression of therapeutic proteins. This article will focus on a novel transposon system for stable clone development with low variation.

Engineering 105

Engineering Molecular Biology Protein Expression Medical Schools 105

Broad Institute

SEPTEMBER 5, 2024

In recent years, increasingly sensitive mass spectrometers have paved the way for enormous progress: scientists are now able to detect and analyze nearly all proteins expressed in a sample, including ones present only in miniscule amounts.

Disease 85

Disease Protein Expression Engineering Treatment 85

Alta Sciences

APRIL 17, 2024

In this blog article, we review some of these areas of investigation where Altasciences has robust expertise and solution offerings. According to a 2022 article published in Molecular Psychiatry, treatment resistance affects 20 to 60% of patients with psychiatric disorders. Asia, and Europe.

Drug Development 52

Drug Development Treatment FDA Approval Drugs 52

Drug Target Review

JULY 5, 2023

A good introduction to the use of CHO cells for biotherapeutics production can be found in an article commemorating the 20th anniversary of the approval of a product produced using CHO cells. 1 Another critical component in this first step is the selectable marker that will be inserted into the genome along with the GOIs.

Engineering 105

Engineering DNA Laboratories Production 105

The Anticancer Fund

SEPTEMBER 29, 2021

Being listed is no endorsement of the results and conclusions of the article. All articles need to be critically assessed and viewed in their broader research context. Data from patients in the clinical trial showed decreased HER2 total protein expression and phosphorylation of AKT and S6 proteins in tumours.

Clinical Trials 40

Clinical Trials Drugs Trials Protein Expression 40

The Pharma Data

JANUARY 15, 2021

3 HER2 is a tyrosine kinase receptor growth-promoting protein expressed on the surface of many types of tumors including breast, gastric, lung and colorectal cancer. Related Articles: Enhertu (fam-trastuzumab deruxtecan-nxki) FDA Approval History. 2,5 In the U.S., Approximately one in five gastric cancers are HER2 positive.3

Treatment 52

Treatment Trials Disease Therapies 52

Codon

APRIL 9, 2023

AI + Bio *Transfer learning for cross-context prediction of protein expression from 5’UTR sequence. No company makes them for commercial sale in those countries, and hospitals and pharmacies don’t stock them,” writes Maryn McKenna in a recent WIRED article. Biology is the world’s most advanced technology.

Therapies 52

Therapies RNA Engineering Vaccine 52

Drug Target Review

JANUARY 20, 2025

This article explores how innovations in precision medicine are reshaping clinical trials, followed by a discussion on Project Optimus and its impact on dose optimisation. Molecular factors Molecular markers, such as protein expression and signalling pathway activation, also guide therapy choices.

Clinical Trials 52

Clinical Trials Trials Drugs Drug Development 52

Codon

FEBRUARY 16, 2025

This article is best experienced on our website. This repressilator generates a pulse of fluorescent-protein expression just once every 14 generations. In the late 1990s, a young physicist named Michael Elowitz decided to “program” living cells. Assuming a cell-cycle time of 1 hour, it would take around 7.5

DNA 80

DNA RNA Engineering Small Molecule 80