Chemical Biology and Drug Design

DECEMBER 20, 2023

The protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), matrix metalloproteinase (MMP)-2, and MMP-9 were detected by western blotting. Besides, the decreased Nrf2 expression reduced the protein expression levels of MMP-2 and MMP-9 ( p  < .05).

Protein Expression 100

Protein Expression Treatment Drugs Research 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

Therefore, based on ADMET properties, eight optimal natural multi-targets NIs glycosides compounds (IC 50  = 0.094–97.275 μM) are found from radix glycyrrhizae, flos sophorae, caulis spatholobi, radix astragali, radix glycyrrhizae, semen astragali complanati , and common fenugreek seed through network pharmacology, molecular docking, dynamics simulation, (..)

Virus 100

Virus Protein Expression Vaccine Research 100

Chemical Biology and Drug Design

JUNE 21, 2023

The results showed that hsa-miR-503-5p showed high expression, while its target gene CTDSPL presented decreased expression in LUAD. Hsa-miR-503-5p also had high expression in cisplatin-resistant LUAD cells. Binding relationship between the two genes was verified by dual-luciferase reporter assay.

Regulations 100

Regulations Protein Expression Bioinformatics Drugs 100

Chemical Biology and Drug Design

FEBRUARY 19, 2024

SIRT1 expression was enhanced by Icariin, and its knockdown suppressed Icariin-induced BMSC osteogenic differentiation. Moreover, deubiquitinating enzyme USP47 could stabilize SIRT1 protein expression.

Regulations 100

Regulations Protein Expression Research 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

This research is powered to probe whether the molecular mechanism of JAS for IS treatment is coupled with microglia polarization. The mRNA or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real-time polymerase chain reaction or western blot.

Regulations 100

Regulations Protein Expression Treatment Research 100

Chemical Biology and Drug Design

MARCH 5, 2024

The results showed that CA-COS inhibited nitric oxide (NO) production and downregulated the gene expression of nitric oxide synthase (iNOS), and cytokines such as tumor necrosis factor-alpha (TNF-α), IL-1β, and IL-6 without cytotoxic effect.

Protein Expression 100

Protein Expression Therapies Production Disease 100

Chemical Biology and Drug Design

FEBRUARY 15, 2024

RT-qPCR and western blotting were used to test the mRNA and protein expression levels of IL-17 and retinoid-related orphan receptor-γt (RORγt). PGE2 was highly expressed in the DE mouse model. The mRNA and protein levels of IL-17 and the key Th17 transcription factor RORγt were increased in tissues of the DE mice.

Disease 100

Disease Protein Expression Regulations Treatment 100

Chemical Biology and Drug Design

DECEMBER 6, 2023

To sum up, guided by network pharmacological analysis and previous literature research, a p53 interfering cell model has been established with the help of shRNA interference technology to investigate the anti-HS effect of SP-FC dependent on p53 in this study.

Molecular Biology 100

Molecular Biology Protein Expression Treatment Regulations 100

Chemical Biology and Drug Design

AUGUST 23, 2023

We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. The protein expressions were detected via western blot. Ligustrazine at doses of 100 and 1000 μg/mL was administrated in Kupffer cells isolated from THS rats.

Protein Expression 100

Protein Expression Protein Production Treatment Production 100

Chemical Biology and Drug Design

AUGUST 21, 2023

Further, the study detected the effect of SA on cell apoptosis, lipid peroxidation, Fe 2+ level, and ferroptosis-related proteins expression. Finally, the effect of HMGB1 expression on SA in H/R stimulation was studied.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

SEPTEMBER 21, 2023

Colonic pathological changes were analyzed by hematoxylin–eosin staining, and inflammatory factor expressions in serum were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and western blot were performed to quantify ferroptosis-related protein expressions.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 2, 2023

Linagliptin administered to the type 1 diabetic mouse heart significantly reduced the expression levels of the total and cleaved forms of ATF6, ATF4, and p-JNK, caspase 3. According to ELISA findings, TUDCA was more effective in reducing NOX 1 and MDA levels than linagliptin.

Protein Expression 100

Protein Expression Therapies Treatment Research 100

Chemical Biology and Drug Design

AUGUST 20, 2023

Hederagenin attenuated HG-induced increase in mRNA and protein expression of NLRP3, ASC, and IL-1β. The secretion levels of fibrosis-related biomarkers were analyzed by ELISA. Results showed that hederagenin reduced HG-induced proliferation increase in HRMCs and HRPTEpiCs. IV, PAI-1, and TGF-β1.

Bioinformatics 100

Bioinformatics Protein Expression Research 100

Chemical Biology and Drug Design

AUGUST 9, 2023

A label-free proteomics technology was employed to investigate alterations in protein expression in LoVo cells treated with plumbagin. Cell cycle analysis and cell apoptosis analysis were conducted to break down the anticancer impact of plumbagin on LoVo cells.

Bioinformatics 100

Bioinformatics Protein Expression Treatment Research 100

Chemical Biology and Drug Design

APRIL 15, 2023

The mRNA and protein expression levels of p53, p-glycoprotein (P-gp), ATM, ATR, CHK1, and CHK2 were assessed through qRT-PCR and western blotting. OECM-1 and OECM-1/PTX were transfected with miR-34 mimic and inhibitor. Cellular proliferation and apoptosis were evaluated through MTT assay and flow cytometry, respectively.

DNA 100

DNA Protein Expression Research 100

Chemical Biology and Drug Design

JUNE 8, 2023

In addition, SAS treatment caused a significant decrease in the mRNA and protein expression of xCT and GPX4, and a significant increase in ACSL4 expression in TE-1 cells treated with SAS. Flow cytometry results showed that the ferroptosis level was significantly increased after SAS treatment.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

SEPTEMBER 25, 2023

Also, Vinp downregulated α-Syn protein expression and MDA level, while upregulated SOD activity in the striatum of PD rats. Vinp treatment increased the horizontal movement frequency and number of squares crossed, reduced the contact time, and rotation frequency in PD rats.

Disease 100

Disease Protein Expression Treatment Research 100

Chemical Biology and Drug Design

DECEMBER 8, 2023

BoNT/A (1 nM) attenuated LPS/ATP-stimulated inhibition of viability and CAMP expression and upregulation of inflammatory mediators, pyroptosis-related proteins, and ELANE expression in rat DRG neurons, which was counteracted by CAMP silencing.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Elisa's result showed that CQQNC can significantly decrease the expression levels of cPLA2, sPLA2, PGE2, cAMP, and 15-PGDH after stimulating IL-1β to bEnd.3 3 cells in a dose-dependent manner ( p  < .01, 01, p  < .001, 001, p  < .0001). 3 ( p  < .0001, 0001, p  < .001, 001, p  < .01).

Treatment 100

Treatment Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 4, 2024

The expression of miR-181a-3p was inhibited; however, SHQ1 expression was increased by β-sitosterol treatment of A549/anlotinib cells. The inhibition of SHQ1, ATF6, and GRP78 protein expression by β-sitosterol in A549/anlotinib cells was rescued by increased miR-181a-3p.

Protein Expression 100

Protein Expression Regulations Treatment Drugs 100

Drug Target Review

MARCH 15, 2024

Efforts have focused on Chinese hamster ovary (CHO) cell line improvements since it is a commonly used host for the expression of therapeutic proteins. This article will focus on a novel transposon system for stable clone development with low variation. Disclaimer: For research use only.

Engineering 105

Engineering Molecular Biology Protein Expression Medical Schools 105

Chemical Biology and Drug Design

DECEMBER 15, 2023

μM), significantly and dose-dependently induced apoptosis of SCC-12 and SK-MEL-28 cells, as evidenced by the suppression of Bcl-2 and upregulation of Bax, cleaved caspase-3, caspase-9, and PARP protein expression levels. The most active compounds 11 (A431: IC 50  = 5.0 μM, μM, SCC-12: IC 50  = 2.9 μM, μM, SKMEL-28: IC 50  = 4.9 μM,

Small Molecule 100

Small Molecule Protein Expression Laboratories Research 100

Alta Sciences

APRIL 17, 2024

Five Promising Treatment Areas in Early-Phase Drug Development in 2024 aasimakopoulos Wed, 04/17/2024 - 15:52 Early-phase drug development is an ever-changing landscape, as emerging science leads to new promising areas of research for the treatment of human health issues. Asia, and Europe.

Drug Development 52

Drug Development Treatment FDA Approval Therapies 52

Broad Institute

SEPTEMBER 5, 2024

In recent years, increasingly sensitive mass spectrometers have paved the way for enormous progress: scientists are now able to detect and analyze nearly all proteins expressed in a sample, including ones present only in miniscule amounts.

Disease 80

Disease Protein Expression Engineering Treatment 80

The Anticancer Fund

SEPTEMBER 29, 2021

Our intention is to help bring these findings to the attention of the broader cancer research community. Being listed is no endorsement of the results and conclusions of the article. All articles need to be critically assessed and viewed in their broader research context.

Clinical Trials 40

Clinical Trials Drugs Trials Protein Expression 40

Codon

APRIL 9, 2023

This is Codon Digest, my weekly roundup of research, news, and industry highlights about the future of biology. AI + Bio *Transfer learning for cross-context prediction of protein expression from 5’UTR sequence. Nucleic Acids Research. Nucleic Acids Research. Nucleic Acids Research. Nature Methods.

Therapies 52

Therapies RNA Engineering Vaccine 52

The Pharma Data

JANUARY 15, 2021

for the treatment of patients with metastatic gastric cancer, and represents a major advance in managing this difficult-to-treat disease,” said Antoine Yver, MD, MSc, Executive Vice President and Global Head, Oncology Research and Development, Daiichi Sankyo. Related Articles: Enhertu (fam-trastuzumab deruxtecan-nxki) FDA Approval History.

Treatment 52

Treatment Trials Disease Therapies 52