Drug Target Review

JANUARY 2, 2024

Forging the toolkit for subcellular omics Cellular components known as organelles, consisting of RNA and protein, exert vital influences on human wellbeing and ailments by sustaining equilibrium, managing growth and ageing, and producing energy.

RNA 128

RNA Immune Response Therapies Disease 128

KIF1A

JANUARY 20, 2024

In this week’s article, researchers in Shanghai, China identified 6 children with KIF1A mutations and compared their symptoms to cases in the literature. Instead, this is what is called a splicing mutation; it occurs at the boundary between coding DNA that is read into RNA and protein, and noncoding DNA that is excluded.

DNA 105

DNA RNA Disease Research 105

Chemical Biology and Drug Design

JUNE 11, 2024

Using RNA-seq and through in vitro and in vivo studies, we determined that brusatol suppresses hepatocellular carcinoma progression by inducing ATF3-mediated ferroptosis. RNA sequencing (RNA-seq) and bioinformatics analyses of HCC cells were utilized to elucidate the mechanism underlying the effects of BRU in HCC.

RNA 100

RNA Bioinformatics Treatment Therapies 100

Chemical Biology and Drug Design

APRIL 1, 2023

Berberine anticancer properties are demonstrated due to the interaction of berberine with micro-RNA. The summarized information presented in this review article may help and lead the researchers, scientists/industry persons to use berberine as a promising candidate against cancer.

Therapies 100

Therapies RNA Regulations Research 100

Chemical Biology and Drug Design

OCTOBER 27, 2023

Circular RNAs (circRNAs) are a unique kind of non-coding RNA and play an important regulatory role in OP. RNA immunoprecipitation (RIP) and RNA pull-down assays were performed to validate the binding relationship among circ_0005753, PTBP1, and TXNIP.

RNA 100

RNA Treatment Regulations Research 100

PLOS: DNA Science

MAY 16, 2024

As soon as the initial RNA genome sequence of the pathogen that would be named SARS-CoV-2 was published, CDC and other organizations began developing diagnostic tests. After sequencing, artificial intelligence adds context to RNA and DNA sequences through comparisons to others. Work on vaccines ensued too.

Virus 98

Virus DNA RNA Vaccine 98

KIF1A

SEPTEMBER 9, 2023

To investigate these proteins, the authors used RNA interference to silence Nup153 and KIF1A. Read the Article Rare Roundup A broad genetic test saved one newborn’s life. Read the Article The post #ScienceSaturday: September 9, 2023 appeared first on KIF1A. What’s a pre-print? Check out this #ScienceSaturday post to learn more.

RNA 105

RNA Treatment Research Disease 105

Codon

AUGUST 26, 2024

Sign up to receive new articles from Asimov Press. Remdesivir and other broad-spectrum antivirals work by jamming up RNA-dependent RNA polymerase (RdRp), an enzyme that nearly all RNA viruses use to replicate their genomes. Nor was remdesivir as effective as people hoped. Unsubscribe any time.

RNA 97

RNA Virus DNA Vaccine 97

KIF1A

SEPTEMBER 16, 2023

Note: For simplicity I will use the term KIF1A when referring to UNC-104 Post-translational modifications: When we talk about modifications to proteins, it’s usually in the context of mutations – a modification to the KIF1A gene makes altered RNA, which translates into altered KIF1A protein. What’s a pre-print?

Regulations 105

Regulations RNA Disease Research 105

Chemical Biology and Drug Design

NOVEMBER 13, 2023

However, it is not clear whether propofol mediates OSCC progression through regulating circular RNA (circRNA) network. RNA interaction was confirmed by dual-luciferase reporter assay and RIP assay. Abstract Propofol has been shown to inhibit oral squamous cell carcinoma (OSCC) progression.

RNA 100

RNA Regulations Research 100

Drug Target Review

APRIL 7, 2025

Haemoglobin A1c (HbA1c) is a validated surrogate endpoint for the reduction of microvascular complications associated with diabetes mellitus; reduced HIV-RNA levels serve as an endpoint for HIV disease control; and a reduction in low-density lipoprotein (LDL) cholesterol is used as an endpoint indicating lower likelihood of cardiovascular events.

Clinical Development 59

Clinical Development Clinical Trials Clinical Pharmacology Trials 59

Chemical Biology and Drug Design

JANUARY 23, 2024

A gradient of emodin and long noncoding RNA HCP5 overexpression plasmid/small interfering RNA targeting HCP5 (siHCP5) were used for treating CRC cells. Expression levels of RNAs and proteins were detected by utilizing quantitative real-time polymerase chain reaction and Western blot.

RNA 100

RNA Regulations Research 100

Chemical Biology and Drug Design

AUGUST 7, 2023

RNA sequencing (RNA-seq) analysis identified 18 differentially expressed genes (DEGs) between D-galactose-treated and PPS-pretreated mice abdominal aorta.

RNA 100

RNA Regulations Research 100

Chemical Biology and Drug Design

DECEMBER 28, 2023

The prognostic value of long noncoding RNA (lncRNA) LINC00460 has been reported in cervical cancer. The interaction between miR-338-3p and LINC00460 or transforming growth factor β-induced protein (TGFBI) was confirmed by RNA immunoprecipitation (RIP) and luciferase reporter assays.

Bioinformatics 100

Bioinformatics RNA Regulations Drugs 100

Chemical Biology and Drug Design

JULY 27, 2023

cells induced by poly (I:C) were treated with or without PA, and then RNA-sequencing, GO and KEGG analysis were carried out to obtain the five differentially expressed genes (IL-1β, HMOX1, PTGS2, Abca1, and P65), which were verified by QRT-PCR and western blot. RNA-sequencing was applied to analyze the differentially expressed genes (DEGs).

RNA 100

RNA Virus Disease Drugs 100

KIF1A

MAY 4, 2024

In this week’s article, researchers in Brazil identified a KIF1A mutation in a cohort of patients with congenital ataxia. Later, she developed an interest in the RNA world, specifically focusing on alternative splicing and its correlation with neuron cell properties within and across cell types.

Science 116

Science Medical Schools Research RNA 116

Alta Sciences

FEBRUARY 19, 2025

An oligonucleotide is a short strand of nucleotides, the building blocks of DNA or RNA, used in genetic research and therapy. Our experts stay up to date on all new and evolving regulations to guarantee regulatory compliance in your studies. Have questions about your oligonucleotide therapeutic studies? Get in touch with our experts today.

Drug Development 52

Drug Development Clinical Pharmacology Immune Response Drugs 52

Chemical Biology and Drug Design

JANUARY 4, 2024

Meanwhile, circular RNA (circRNA) circ_0001615 has been reported to be involved in the malignant development of CRC. The binding between miR-873-5p and circ_0001615, or LASP1, was predicted by Starbase, followed by verification by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays.

Regulations 100

Regulations RNA Treatment Research 100

KIF1A

JULY 29, 2023

Genetic and RNA-based therapies seek to correct mutant KIF1A – improving its force generation, velocity, and distance traveled by increasing the ratio of healthy to mutant KIF1A. Read the Article The post #ScienceSaturday: July 29, 2023 appeared first on KIF1A.

RNA 98

RNA Clinical Trials Disease Science 98

Drug Target Review

MAY 24, 2024

More explanation and practical tips for using these technologies will be explored in future articles. 2 AlphaFold 3 has been built to model DNA, RNA and smaller molecules (ligands). These are foundational to drug discovery and will be discussed in future articles. Alphafold 3.0: the AI protein predictor gets an upgrade.

RNA 59

RNA DNA Bioinformatics Science 59

Codon

DECEMBER 12, 2024

” The report accompanies an article in Science , also released today, entitled “Confronting Risks of Mirror Life.” For the article in Science , more than 30 scientists from ten countries are calling on the broader community to confront the risks of mirrored life. ” But what, exactly, is a mirrored organism?

RNA 102

RNA DNA Vaccine Virus 102

Chemical Biology and Drug Design

DECEMBER 28, 2023

RNA sequencing analysis was conducted to study the gene expression profiles of Formononetin-induced H1975_OR cells. However, the potentials and the mechanisms of Formononetin to counteract the Osimertinib resistance in NSCLC are unclear.

RNA 100

RNA Regulations Treatment Research 100

Drug Target Review

AUGUST 28, 2024

We then incubate the cells to mature into ‘pro-regenerative’ macrophages and insert two RNA messages to produce two human proteins that will be expressed at the site of injury when these ‘engineered’ macrophages are given back to the patient. The patient’s own monocytes, a type of white blood cell, are removed via an apheresis procedure.

Therapies 116

Therapies Disease Engineering Medical Schools 116

Chemical Biology and Drug Design

FEBRUARY 20, 2024

Abstract N6-methyladenosine (m6A) modification and m6A-related RNA-binding proteins (RBPs) play vital roles in various aspects of circRNA metabolism. Hsa_circRNA_103820, downregulated by IGF2BP3, could encode a 188-aa peptide and inhibited lung cancer cell malignant progression by inhibiting the AKT pathway.

Bioinformatics 100

Bioinformatics RNA Regulations Research 100

Chemical Biology and Drug Design

JANUARY 4, 2024

The differentially expressed genes (DEGs) were identified by RNA-seq and subjected to GO and KEGG pathway analyses. The mouse model of ammonia-induced lung fibrosis was established, and the role of PLD was assessed by H&E and Masson's trichrome staining. BEAS-2B cells were treated with NH 4 Cl alone or along with PLD.

RNA 100

RNA Disease Research 100

Chemical Biology and Drug Design

JANUARY 23, 2024

CyclinD1, MMP2, RNA-binding motif protein 3 (RBM3), embryonic lethal abnormal visual-like protein 1 (ELAVL1), Wnt3A, and β-catenin protein levels were assessed using western blot. RBM3 and ELAVL1 expression was measured using real-time quantitative polymerase chain reaction (RT-qPCR).

Regulations 100

Regulations RNA Treatment Research 100

Chemical Biology and Drug Design

NOVEMBER 27, 2023

To clarify how A6 affected on MCF-7 cells, RNA-seq analysis was conducted to evaluate the whole genome effect of A6 on gene expression. A6 significantly disrupted the amino acid metabolism and inhibited migration of MCF-7. In addition, A6 induced apoptosis of MCF-7 cells.

Bioinformatics 100

Bioinformatics RNA Regulations Therapies 100

Chemical Biology and Drug Design

JULY 26, 2023

Circular RNA (circRNA) is an important regulator of gastric cancer progression. Sino suppressed GC cell proliferation, migration, invasion, and promoted apoptosis via regulating the circTRPM7/miR-145-5p/PBX3 axis. Abstract Sinomenine has been found to have antitumor effects in a variety of cancers, including gastric cancer.

Regulations 100

Regulations RNA Research 100

Chemical Biology and Drug Design

MARCH 8, 2024

The binding between miR-653-5p and circ_0139658 or Yin Yang 1 (YY1), was verified using dual-luciferase reporter and RNA pull-down assays. Cell proliferation, apoptosis, invasion, migration, as well as inflammatory response were evaluated by CCK-8 assay, EdU assay, flow cytometry, transwell assay, and ELISA analysis, respectively.

Regulations 100

Regulations RNA Treatment Research 100

Chemical Biology and Drug Design

JANUARY 24, 2024

MiR-556-3p and Acyl-CoA synthetase long-chain family member 4 (ACSL4) interaction was confirmed via dual-luciferase reporter assay and RNA pull-down assay. The expression levels of microRNA (miR)-556-3p and ACSL4 were tested via quantitative real-time PCR.

Regulations 100

Regulations RNA Treatment Research 100

Chemical Biology and Drug Design

DECEMBER 1, 2023

circinteractome, and CircBank and validated using dual-luciferase reporter and RNA pull-down assays. The molecular mechanisms related to circ_0068464 were predicted through online prediction websites Starbase 2.0, The tumorigenic ability of BD and circ_0068464 on CRC was confirmed by xenograft experiments.

RNA 100

RNA Regulations Treatment Drugs 100

Drug Target Review

AUGUST 9, 2023

Published on bit.bio’s website, this article authored by Dr Tony Oosterveen dives into the close relationship between FTD and ALS with a focus on mechanisms underlying the neuropathology of both diseases. 14 TDP-43 is a DNA and RNA binding protein that regulates the expression and splicing of several target transcripts.

RNA 98

RNA Regulations Disease DNA 98

Chemical Biology and Drug Design

SEPTEMBER 15, 2023

CRS rabbit models were constructed by Staphylococcus aureus infection and rabbits were injected with lentiviral vectors of short hairpin RNA-targeting Chemerin (shChemerin), followed by micro-computed tomography (CT) scan. Patients suffering from CRS without/with nasal polyps (CRSsNP/CRSwNP), along with volunteers, were recruited.

RNA 100

RNA Treatment Disease Research 100

Chemical Biology and Drug Design

FEBRUARY 26, 2024

The m 6 A-RNA immunoprecipitation (MeRIP) assay detected the m 6 A modification profile. Oxidative stress was analyzed by detecting the contents of reactive oxygen species (ROS), superoxide dismutase (SOD) and malondialdehyde (MDA). The binding of miR-4654 and SOD2 was confirmed by dual-luciferase reporter assay.

RNA 100

RNA Regulations Therapies Research 100

Chemical Biology and Drug Design

AUGUST 24, 2023

When the expression of TGFBR3 on the surface of tumor cells (HeLa) was knocked down by RNA interference, the high delivery efficiency of T3BP was correspondingly reduced by 40%, indicating that the delivery was TGFBR3-dependent.

RNA 100

RNA Therapies Drugs Treatment 100

KIF1A

FEBRUARY 4, 2024

In this week’s article, researchers in Japan performed whole genome sequencing on 315 patients who were enrolled in the HAM patient registry. We each have two DNA copies of the KIF1A gene; each copy is called an allele and makes its own RNA, which is then translated into protein. What is an allele specific ASO?

RNA 52

RNA DNA Disease Trials 52

PLOS: DNA Science

MAY 25, 2023

Now Avidity Biosciences has developed a candidate drug that weds a monoclonal antibody (MAb) to a short piece of RNA – a small interfering RNA (siRNA). This novel class of RNA therapeutics is called Antibody Oligonucleotide Conjugates (AOCs™). Synthesizing a bit of RNA, Levin writes, is the simple part.

Treatment 52

Treatment DNA RNA Clinical Trials 52