Molecular Design

DECEMBER 19, 2023

Regardless of whether binding to target is covalent or non-covalent, the concentration and affinity of substrates (as well as cofactors such as ATP) need be properly accounted for when interpreting effects of chemical probes in cell-based assays. As measurement of k.inact/K.i values for a covalent chemical probe.]

Small Molecule 72

Small Molecule Biochemical Assays Cell Based Assays Research 72

Agency IQ

APRIL 12, 2024

For example, in describing how potency should be demonstrated for CGT products, the 2011 guidance simply stated that potency for gene therapy products is derived from “at least two biological activities for its potency: the ability to transfer a genetic sequence to a cell; and the biological effect of the expressed genetic sequence.”

Production 40

Production FDA Therapies Biochemical Assays 40

Drug Discovery World

OCTOBER 14, 2022

Trapped ion mobility spectrometry: Improving the specificity of MALDI-MS-based HTS . Büttner, MALDI-TOF mass spectrometry-based high-throughput screening for inhibitors of the cytosolic DNA sensor cGAS, SLAS Discovery, 2020, 25: 372–383, [link]. Weigt, C.A. Parrish, J.A. Krueger, C.A. Oleykowski, A.R. Rendina and C. Segarra-Fas, A.P.

Synthetic Chemistry 130

Synthetic Chemistry Cell Based Assays Biochemical Assays Analytical Chemistry 130