CDD Vault Update (February #2 2025): Advanced Macromolecules - Modified Bioconjugates Support in CDD Vault
Collaborative Drug
FEBRUARY 21, 2025
CDD Update February 2025 #2 New Macromolecule Mode Edit DNA, RNA & Peptides with Bioconjugate Support
Drug Discovery Industry Roundup with Barry Bunin — December 18, 2023
Collaborative Drug
DECEMBER 18, 2023
Discover the impact of 'junk DNA' on cancer, teen obesity treatments, Neanderthal genes on sleep patterns, and Nobel discoveries shaping medicine
DNA 
298
298 Join 15,000+
Insiders
Sign Up for our Newsletter
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Drug Discovery Industry Roundup with Barry Bunin — July, 22 2024
Collaborative Drug
JULY 22, 2024
DNA Discovery that Could Help Find Pancreatic Cancer Cure. Is Revolutionizing Drug Development. Holy Grail for Weight-Loss Drugs Is Sleep Apnea?
DNA 263
263 
CRISPR 101: Cytosine and Adenine Base Editors
addgene Blog
FEBRUARY 13, 2025
Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair (HDR) , the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ) , HDR occurs at a relatively low frequency, especially in nondividing cells.
DNA 105
105 
Plasmids 101: Modular Cloning Applications and Kits
addgene Blog
MAY 14, 2024
Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. In this blog we will review common uses of MoClo and the kits Addgene offers to kickstart these applications, no matter what your model organism is.
DNA 105
105 DNA 105
105 
The Love Songs of W. E. B. Du Bois Celebrates Afro-Indigenous History with Genealogy – No DNA Needed
PLOS: DNA Science
MAY 18, 2023
Perhaps more telling, I recently discovered a previously unknown branch of my own family tree that includes a dozen half-siblings that I know of, from a DNA test that I didn’t even remember taking. I told my story in a New York Times Modern Love podcast and in blog posts and articles. Which one was our biodad?
DNA 92
92 
Degrading DNA with Cascade-Cas3
addgene Blog
FEBRUARY 11, 2025
The versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors to light.
DNA 59
59 DNA 59
59 
CRISPR 101: Off-Target Effects
addgene Blog
FEBRUARY 8, 2024
For CRISPR work, this often means DNA cleavage at unanticipated sites. In this blog, we will discuss when off-targets can be an issue in CRISPR, how to predict and minimize them, and ways to control for and quantify them when they can’t be avoided.
DNA 102
102 DNA 102
102 
AI-Designed Enzymes
Codon
FEBRUARY 13, 2025
They synthesized DNA encoding each protein variant, expressed the proteins in E. He also writes on a blog focused on the intersection of biology and AI at owlposting.com. Achieving a non-zero turnover rate at all is something that nobody has ever done before with enzymes as complex as these.
Small Molecule 124
124 
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
addgene Blog
SEPTEMBER 12, 2023
DNA sequence analysis. Primer design. Plasmid mapping. We all have our favorite tools for tackling these particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology tools all in one place.
DNA Analysis Finds New Target for Diabetes Drugs
NIH Director's Blog: Drug Development
MARCH 11, 2014
Credit: Jane Ades, National Human Genome Research Institute, NIH Type 2 diabetes (T2D) tends to run in families, and over the last five years the application of genomic technologies has led to discovery of more than 60 specific DNA variants that contribute to risk.
DNA 52
52 
DNA Barcodes Could Streamline Search for New Drugs to Combat Cancer
NIH Director's Blog: Drug Discovery
MARCH 8, 2016
Instead of the black, printed stripes of the Universal Product Codes (UPCs) that we see on everything from package deliveries to clothing tags, they used short, unique snippets of DNA to label cells. DNA barcoding has already empowered single-cell analysis, including for nerve cells in the brain. PRISM consists of two key components.
DNA 52
52 
Plasmids 101: Stringent Regulation of Replication
addgene Blog
JUNE 20, 2024
As described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or may not be synchronized with the replication of the host cell's chromosomal DNA. All plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent.
Regulations 93
93 
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
addgene Blog
JUNE 25, 2024
Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019; Chen & Liu, 2023).
DNA 98
98 
Build Your CRISPR Vocabulary
addgene Blog
JULY 2, 2024
We’ll cover the common terminology for the proteins, DNA, and RNA used in CRISPR. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you.
RNA 103
103 
Why I’m Not Worried that ChatGPT Will Replace Me as a Biology Textbook Author
PLOS: DNA Science
SEPTEMBER 7, 2023
As genetics morphed into genomics, artificial intelligence stepped in, layering the combinatorial information of comparative genomics onto DNA sequences. Train an algorithm on the DNA sequences of a known disease-causing gene, then search for identical or highly similar sequences in cells from other individuals to assist diagnosis.
DNA 98
98 
Optical density and absorbance measurements | BMG LABTECH
BMG Labtech
DECEMBER 20, 2024
Whether it is determining concentrations of molecules like proteins or DNA, looking at enzyme kinetics for crucial reactions, or measuring something as fundamental as cell growth, you will find references to absorbance or optical density measurements. But how do they relate to each other? Lets start by looking at some definitions.
DNA 52
52 
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
addgene Blog
SEPTEMBER 26, 2024
coli , SLIC is a cheap, standardized, and rapid multi-part DNA assembly method. Not only does this system not use site-specific recombination, it also doesn’t require a ligation step. Based on the robust system of homologous recombination found in E. Read on to learn how to use it in your research!
DNA 94
94 
Antibodies 101: Producing Recombinant Antibodies
addgene Blog
AUGUST 13, 2024
Since recombinant antibodies conveniently allow for unlimited production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in your own experiments. It is possible to produce your own recombinant antibodies with some molecular biology and cell culture experience.
A Protein Printer
Codon
JULY 11, 2023
That’s because proteins are made, in the laboratory, using synthetic DNA and cells; and DNA is expensive. Our machine would make proteins without using any DNA or cells. All cells make proteins in two steps: DNA is transcribed into messenger RNA, which is then translated into protein.
RNA 71
71 
Cave Coronavirus in Wuhan Lab Seeded COVID – The Truth Has Always Been Out There, in the Genetics
PLOS: DNA Science
JUNE 13, 2024
Experiments at the Wuhan facility were done at the lowest two of the four standard biosafety levels, which were established at the dawn of recombinant DNA research in the 1970s. The Likely Predecessor of SARS-CoV-2 – From my Past Blog Posts I wrote 100+ DNA Science blog posts during the pandemic about the virus.
Virus 52
52 
Next Generation Sequencing (NGS): why is quantifying nucleic acids important? | BMG LABTECH
BMG Labtech
OCTOBER 4, 2023
These numbers reflect impressive developments in DNA sequencing technologies but in most cases represent the immediate costs of consumables on the sequencing instrument. 2 DNA sequencing is also reliant on sample processing and DNA library preparation before sequencing as well as bioinformatics and data analysis after sequencing.
DNA 52
52 
Genomic Sequencing Paves the Way for Better Antiviral Treatments
PerkinElmer
JULY 13, 2020
Sequencing viral genome isolates and creating phylogenetic trees Sequencing a viral genome requires isolating the viral RNA, reverse transcribing it into DNA, and then performing multiple amplification steps using primers. Finally, the DNA library is sequenced, the sequences are aligned, and the full genome annotated.
Treatment 52
52 
Traditional Meat Industry’s Beef With Alternative Protein Continues with the FAIR on Labels Act
FDA Law Blog: Biosimilars
MARCH 5, 2024
Gaulkin & Riëtte van Laack — As readers of this blog know, there is a lot of contention about the naming of alternative protein products (APPs), including both plant-based and cell-cultured alternatives for (traditional) animal products. We’ve previously blogged about this ongoing battle here , here , here , and here. By Sophia R.
Fast Biology
Codon
FEBRUARY 20, 2024
Thanks to decades of progress in molecular sequencing technologies, it is simple to read out the order of nucleotides in a DNA sequence, for example, or to quantify messenger RNAs as they are made by a cell. Escherichia coli have about 5 million base pairs of DNA in their genome, and divide every 20 minutes. This paper is one example.
RNA 98
98 
Models of Life
Codon
SEPTEMBER 29, 2024
mRNA was the intermediate stage between DNA and protein, a dynamic entity that shifted depending on the second-to-second needs of the cell, able to point out if a cell was cancerous or stressed, what kind of cell it was, and so on. He also writes for a blog focused on the intersection of biology and AI at owlposting.com.
Engineering 141
141 
Codon Digest: Agents Design Proteins
Codon
MAY 22, 2023
And experiments at Tune Therapeutics showed that CRISPR epigenome editing — which doesn’t cut DNA at all, but merely silences genes by adding chemical groups to them — can curb “bad cholesterol” by more than 50% in monkeys. These tools do not cut or nick DNA, and so they may be safer than other options.
DNA 57
57 
EIDD-2173, ATI-2173, Fosclevudine alafenamide
New Drug Approvals
AUGUST 22, 2023
kb relaxed circular DNA (rcDNA) genome that is used to form covalently closed circular DNA (cccDNA) in a host cell. The cccDNA is then transcribed by RNA polymerase II, a host DNA-dependent RNA polymerase, to produce pregenomic RNA (pgRNA). HBV possesses a 3.2- The results are shown below in Tables 8 and 9.
RNA 52
52 
Novel Therapeutic Candidate Puts the STING Back into Immunotherapy
PerkinElmer
MAY 6, 2022
For example, the STimulator of InterferoN Genes (STING) pathway, which is known to play a critical role in innate immunity in response to viral and bacterial DNA, has also been shown to promote anti-tumor immune responses. link] The post Novel Therapeutic Candidate Puts the STING Back into Immunotherapy first appeared on PerkinElmer Blog.
Codon Digest: Discovering Antibiotics with Deep Learning
Codon
MAY 30, 2023
This week: A way to measure a transgene’s expression in the brain using ultrasound, a DNA sequencing method that uses 1000x less reagents, and base editors get even smaller. An engineered version of this protein can convert DNA bases with efficiencies up to 92%. so this Digest will be published more irregularly.
DNA 52
52 
Codon Digest: Discovering Antibiotics with Deep Learning
Codon
MAY 30, 2023
This week: A way to measure a transgene’s expression in the brain using ultrasound, a DNA sequencing method that uses 1000x less reagents, and base editors get even smaller. An engineered version of this protein can convert DNA bases with efficiencies up to 92%. so this Digest will be published more irregularly.
DNA 52
52 
Everything is Cabbage. Now It's Gene-Edited.
Codon
MAY 18, 2023
shelves, it’s easy to find DNA in the air , and some weird insect cybernetics. 2/ Your DNA is Everywhere. A study published Monday in Nature Ecology & Evolution shows that tiny amounts of human DNA, shed into the soil or air, can be easily detected using little more than a $1,000 Nanopore MinION. Read more in WIRED.
DNA 52
52 
Everything is Cabbage. Now It's Gene-Edited.
Codon
MAY 18, 2023
shelves, it’s easy to find DNA in the air , and some weird insect cybernetics. 2/ Your DNA is Everywhere. A study published Monday in Nature Ecology & Evolution shows that tiny amounts of human DNA, shed into the soil or air, can be easily detected using little more than a $1,000 Nanopore MinION. Read more in WIRED.
DNA 52
52 
Codon Digest: Injected Gene Editors
Codon
APRIL 2, 2023
But now, by studying DNA extracted from microbes in the blood of almost 10,000 healthy people, this paper shows that there is no such thing. Read Transcription factors bind to DNA and control gene expression. Read Switchable hydrophobic pockets in DNA protocells enhance chemical conversion. Nature Microbiology. Meeussen J.V.W.
DNA 52
52 
Hey…Walkaway on the Automated Side
PerkinElmer
MAY 7, 2021
One important step of viral, and subsequently vaccine, research is the quantification of viral DNA libraries using plate readers. The post Hey…Walkaway on the Automated Side first appeared on PerkinElmer Blog. Once a viral genome is detected, isolated, multiplied, and purified, it must be quantified and then sequenced.
Vaccine 52
52 
Codon Digest: Agents Design Proteins
Codon
MAY 22, 2023
And experiments at Tune Therapeutics showed that CRISPR epigenome editing — which doesn’t cut DNA at all, but merely silences genes by adding chemical groups to them — can curb “bad cholesterol” by more than 50% in monkeys. These tools do not cut or nick DNA, and so they may be safer than other options.
DNA 52
52 
Analytical Testing Considerations for Gene Therapy Products
The Premier Consulting Blog
MARCH 7, 2024
Depending on the gene therapy vector, microbial systems may be employed to produce vector DNA (such as in adeno associated virus (AAV)), in which case there may be bacterial cell banks and plasmid banks. A more rigorous identity test may sometimes be required, such as DNA sequencing of the entire AAV genome.
Production 52
52 
Viral Vectors 101: An Introduction to AAV
addgene Blog
MAY 30, 2023
Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. This post was contributed by guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik.
Virus 58
58 
A Protein Printer
Codon
JULY 11, 2023
That’s because proteins are made, in the laboratory, using synthetic DNA and cells; and DNA is expensive. Our machine would make proteins without using any DNA or cells. All cells make proteins in two steps: DNA is transcribed into messenger RNA, which is then translated into protein.
RNA 52
52 
Monkey Embryos Grow to 25 Days — No Womb Required
Codon
MAY 15, 2023
3/ Prime Editing Spree Prime editors can change DNA in ways that Cas9 — and even base editors — cannot. Known as a "search-and-replace" gene-editing tool, prime editors can delete or replace DNA up to 10,000 bases in length, or substitute one base for another. Read more in Cell. ( #1 , #2 ) (Video credit: Gong Y.
DNA 52
52 
Monkey Embryos Grow to 25 Days — No Womb Required
Codon
MAY 15, 2023
3/ Prime Editing Spree Prime editors can change DNA in ways that Cas9 — and even base editors — cannot. Known as a "search-and-replace" gene-editing tool, prime editors can delete or replace DNA up to 10,000 bases in length, or substitute one base for another. Read more in Cell. ( #1 , #2 ) (Video credit: Gong Y.
DNA 52
52 
Breaking Down Biomarker Discovery: 5 Points
PerkinElmer
AUGUST 12, 2020
The steps taken during the analytical phase are dependent on the type of biomarker, which can range from DNA, RNA, protein, peptide, biomolecular modification(s), or biochemical pathway(s). The post Breaking Down Biomarker Discovery: 5 Points first appeared on PerkinElmer Blog.
RNA 52
52 
Codon Digest: Seeing Colors After Gene Therapy
Codon
JULY 27, 2023
They’ve just finished sequencing the patient’s genome, but they don’t have “DNA sorting” software. billion units of DNA code are transcribed into more than a hundred volumes, each a thousand pages long, in type so small as to be barely legible.” At least, that’s what every textbook says.
Therapies 52
52 
Codon Digest: Seeing Colors After Gene Therapy
Codon
JULY 27, 2023
They’ve just finished sequencing the patient’s genome, but they don’t have “DNA sorting” software. billion units of DNA code are transcribed into more than a hundred volumes, each a thousand pages long, in type so small as to be barely legible.” At least, that’s what every textbook says.
Therapies 52
52 
Let's personalize your content