This site uses cookies to improve your experience. To help us insure we adhere to various privacy regulations, please select your country/region of residence. If you do not select a country, we will assume you are from the United States. Select your Cookie Settings or view our Privacy Policy and Terms of Use.
Cookie Settings
Cookies and similar technologies are used on this website for proper function of the website, for tracking performance analytics and for marketing purposes. We and some of our third-party providers may use cookie data for various purposes. Please review the cookie settings below and choose your preference.
Used for the proper function of the website
Used for monitoring website traffic and interactions
Cookie Settings
Cookies and similar technologies are used on this website for proper function of the website, for tracking performance analytics and for marketing purposes. We and some of our third-party providers may use cookie data for various purposes. Please review the cookie settings below and choose your preference.
Strictly Necessary: Used for the proper function of the website
Performance/Analytics: Used for monitoring website traffic and interactions
Mirikizumab effectively inhibits the interaction of IL-23 with its receptor, and potently blocks IL-23-induced IL-17 production in cell-basedassays while preserving the function of IL-12. In both local and systemic in vivo mouse models, mirikizumab blocked IL-23-induced keratin mRNA or IL-17 production, respectively.
In cell-basedassays, pegozafermin had a similar receptor engagement profile as native FGF21, with approximately 8-fold higher potency at FGF receptor 1 (FGFR1).
Herein, we used a targeted covalent inhibition strategy and identified a covalent inhibitor, TD19, which selectively inhibits ALKBH5 compared with FTO demethylase in protein-based and tumor cell-basedassays. TD19 irreversibly modifies the residues C100 and C267, preventing ALKBH5 from binding to m6A-containing RNA.
The perennial challenge with STING-seq (and all cell-basedassays) is finding the right cell type for the disease or trait. This encompasses many common diseases and we are optimistic that STING-seq will be a useful platform to rapidly understand which variants and genes are key players in these diseases.
In this blog, we highlight some of the solutions available for researchers to tackle atherosclerotic vascular disease: from fundamental research rooted in ischemia-reperfusion studies and cell-basedassays to drug discovery efforts and the early detection of drug-related side effects. What is atherosclerotic vascular disease?
Image analysis software captures information about more than 3,000 cellular features, providing a deep view into the impacts of genetic variants, small molecule or genetic perturbations, and more on cellular shape, structure, and function. “It moves the community from a very targeted, hypothesis-driven, unidimensional cell-basedassay framework to (..)
All three laboratories across North America are uniformly designed and managed. Whether as a standalone bioanalytical service or as part of an integrated program, Altasciences provides top-quality data for TK, PK, and PD determinations to support preclinical and clinical studies.
Following the high-throughput screening were cell-basedassays, which demonstrated that the identified leads had strong anti-viral action. Of these 10,000 compounds, some were drugs already approved for other uses; others were drug candidates being used in clinical trials. Still others were simply known inhibitors of Mpro.
Dr. Pepe has a PhD in cancer biology and DNA repair and extensive experience in development and validation of cell-basedassays. Alessandra Pepe, AD Processes and Infrastructure Manager at Merck Group.
To gain insight into the use of chemical probes, we conducted a systematic review of 662 publications, understood here as primary research articles, employing eight different chemical probes in cell-based research. [
Finally, ChEMBL version 27 was a special SARS-CoV-2 release focused on large-scale drug screening studies for anti-viral activity, in particular cell-basedassays with well-characterised compounds. Currently, there are ~ 950 K activities for Plasmodium species alone.
CAMBRIDGE, England–( BUSINESS WIRE )– Axol Bioscience , a biotechnology company specializing in the use of stem cell technology to manufacture disease relevant cellbasedassay systems to the drug discovery industry, today announced the appointment of Ross Wheatcroft as the Global Strategic Accounts Manager.
allowing researchers to use “certain alternatives to animal testing, including cell-basedassays and computer models, to obtain an exemption from the FDA to investigate the safety and effectiveness of a drug.”
ChEMBL contains a broad range of binding, functional and ADMET type assays in formats ranging from in vitro single protein assays to anti proliferative cell-basedassays. Some variation is expected, even for very similar assays, since these are often performed by different groups and institutes.
There has been a resurgence of interest in the development of small molecules modulating kinases and of antibodies to cell surface receptors, guided by the selection of molecules having the longest residence time, as this is a key factor to the specificity and real efficacy of new therapeutics.
For example, ELISA and multiplexed assays are used for quantifying proteins or cytokines, ELISpot is ideal for detecting antigen-specific T-cell responses, flow cytometry helps assess cell populations and functions, and cell-basedassays are essential for evaluating the functional activity of immune cells.
The definition goes on to list potential options for such tests: cell-basedassays, organ chips and microphysiological systems, computer modeling, other nonhuman or human biology-based test methods such as bioprinting, as well as animal tests. FDORA § 3209(a)(2).
When combined with CellProfiler (a machine learning-based image profiling application), LipocyteProfiler can reveal information about more than 3,000 cellular features, providing a deep view into the impacts of genetic variants, small molecule or genetic perturbations, and more on cell shape, structure, and function. “It moves the community from (..)
I would argue that the L2021 study has absolutely no relevance whatsoever to the selection of compounds for development since the team will have data available that enables them to rule out the vast majority of the project compounds for nomination.
Data-driven discovery As with many early-stage biotech companies, Five Alarm Bio is highly data-driven, producing vast amounts of experimental data through cell-basedassays and phenotypic screening.
The effects of curcumin, EGCG (epigallocatechin gallate), genistein and resveratrol on four membrane proteins were reported in Ref9 and these effects would raise doubts about activity for any of these compounds (or their close structural analogs) that had been observed in a cell-basedassay.
Regardless of whether binding to target is covalent or non-covalent, the concentration and affinity of substrates (as well as cofactors such as ATP) need be properly accounted for when interpreting effects of chemical probes in cell-basedassays. As measurement of k.inact/K.i
Technology Selection Description of which technology was selected (HPLC, SDS-PAGE, cell-basedassay, ELISA, etc.) and a rationale for selecting it (could be based on development studies, prior knowledge, literature, etc.).
Inspired by her time at GSK, Millie pursued a PhD at the University of Cambridge investigating the signalling pathways that drive cancer cell growth using in-vitro cellbasedassays and biochemical analysis. Driven by an interest in drug discovery Millie joined AstraZeneca after completion of her PhD at the end of 2020.
ASLAN003 is a highly selective and potent inhibitor of human DHODH (IC 50 = 35 nM) and has been shown to be more than 30 times more potent at inhibiting the DHODH enzyme in cell free and cell-basedassays than the first generation inhibitor teriflunomide.
For example, we might examine the structure-activity relationship in a cell-basedassay for consistency with the structure-activity relationship that we’ve observed in the enzyme inhibition assay. We have to make assumptions in these situations and we also need to check that these assumptions are reasonable.
Recombinant monoclonal antibody (mAB) based treatments have proven their efficacy. mRNA vaccines are developing very quickly with dozens of ongoing clinical trials against cancers or infectious diseases (e.g. HIV or more recently SARS-Cov-2).
Different assay organism or cell types: the target protein may be recombinantly expressed in different cell types (the target ID in ChEMBL is assigned based on the original source of the target), or the assays may be run using different cell types.
An active metabolism is a sign of viable cells and can be hence exploited by viability assays (fig.12) In addition, ATP is used as fuel by many luciferase-basedassays.
Cell-basedassays , while more complex to develop and use, offer another option for characterizing product activity at a slightly higher level, allowing sponsors to measure the further downstream effects of products (e.g., morphological cell changes or alterations in protein production or expression).
Endeavor revolutionizes drug discovery by delivering high speed, high resolution, full well scanning of cell-basedassays. Endeavor images 10 times the biology in a fraction of the time, acquiring every cell, every time. This press release features multimedia. View the full release here: [link].
We organize all of the trending information in your field so you don't have to. Join 15,000+ users and stay up to date on the latest articles your peers are reading.
You know about us, now we want to get to know you!
Let's personalize your content
Let's get even more personalized
We recognize your account from another site in our network, please click 'Send Email' below to continue with verifying your account and setting a password.
Let's personalize your content