Drug Target Review

MAY 22, 2024

What are the key findings of Circio’s in vivo proof-of-concept for its circVec circular RNA platform technology compared to conventional mRNA-based expression with DNA vectors? Circular RNA (circRNA) has two major advantages versus mRNA in a vector-expression context. DNA vectors in mouse models?

Therapies 59

Therapies RNA Protein Expression DNA 59

Drug Target Review

JULY 5, 2023

This selectable marker enables the identification of cells which have taken up the GOIs and aids the initial selection of recombinant protein expressing cell lines. They work in the following way: the genes in an expression vector are flanked by inverted terminal repeat sequences (ITRs) to which a specific transposase enzyme can bind.

Engineering 105

Engineering DNA Laboratories Production 105

Broad Institute

AUGUST 24, 2022

Data from standard DNA and RNA sequencing approaches were integrated with mass spectrometry-based proteomics and phosphoproteomic analyses to derive more complete molecular portraits of treatment-responsive versus treatment-resistant tumors. These data suggest a multi-omics predictor for chemotherapy response is within reach.

DNA 52

DNA Protein Expression Treatment Protein Production 52

Conversations in Drug Development Trends

AUGUST 14, 2024

The analysis of circulating tumor DNA (ctDNA) in a liquid biopsy can also permit genotyping and help monitor the effectiveness of chemotherapy. Proteomics Proteomics involves studying the entire set of proteins expressed in a cell or tissue.

Treatment 78

Treatment Therapies Disease Protein Expression 78

DrugBank

JULY 24, 2024

The success of PARP inhibitors in BRCA-mutant ovarian and breast cancers validates this approach, and similar strategies are being tested for other DNA repair enzymes. Indirectly Targeting "Undruggable" Proteins Small molecules can also target "undruggable" proteins indirectly by modulating the production of these proteins.

Small Molecule 83

Small Molecule Drugs Clinical Trials Therapies 83

The Pharma Data

MARCH 30, 2021

Fibroblast activation protein (FAP) is a cell-surface protein expressed at low levels in most normal adult tissues, but over-expressed in common cancers, particularly on cancer-associated fibroblasts that form the tumor stroma, which is essential for growth 1 ,2 ,3 ,4.

Therapies 52

Therapies Licensing Licensing Protein Expression 52

Drug Target Review

MAY 4, 2023

The researchers turned to CRISPR —a gene editing tool that uses “molecular scissors to cut DNA ,” according to Sanjana—to edit the regions identified by GWAS. The researchers then use CRISPR to target each of the regions of the genomes implicated by GWAS and conduct single-cell sequencing to evaluate gene and protein expression.

Disease 52

Disease Small Molecule Protein Expression Research 52

Drug Target Review

SEPTEMBER 10, 2024

The current landscape of protein drug development is characterised by accelerated timelines where new drugs are approved in months rather than years. For example, advances in media and feeds have contributed to higher cell protein expression by optimising nutrient availability and reducing accumulation of waste products within a culture.

Drug Development 64

Drug Development Clinical Supply Production Protein Expression 64

Alta Sciences

APRIL 17, 2024

Several viral vectors are being used currently, in addition to non-viral vectors, such as oligonucleotides, naked DNA, and lipoplexes and polyplexes. They work by binding to specific sequences of nucleotides present within the mRNA structure and can induce mechanisms that either decrease, restore, or modify protein expression.

Drug Development 52

Drug Development Treatment FDA Approval Therapies 52

Codon

APRIL 9, 2023

.” This technology could be used to design protein therapeutics that can bind to, and “shut down,” harmful or misfolded proteins in living cells. Thousands of transcription factors — proteins that bind DNA and control gene expression — were studied in human cells. Bergs A.C.F.

Therapies 52

Therapies RNA Engineering Vaccine 52