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The chemosensitive effects of miR-34 is mediated through increasing DNA damage and apoptosis in a p53 depended manner. The present study aimed to evaluate the effects of miR-34 restoration in OECM-1 oral cancer resistant to paclitaxel (OECM-1/PTX) and its underlying mechanisms through p53-mediated DNA damage and apoptosis.
Biochemical kits was utilized for checking the ATP, mitochondrial DNA, MDA, GSH, and Fe 2+ levels in the Huh7 cells, and western blot for measuring the ferroptosis and AMPK/mTOR related-proteinexpression levels.
To find a DNA fragment that contained the intact ACE2 gene, complete with its embedded regulatory information, they searched libraries of cloned DNA fragments generated as part of the Human Genome Project. The team used the intact human ACE2 gene to increase the chances that mouse cells would incorporate and read the gene correctly.
Related links Merkin Prize Inaugural Merkin Prize in Biomedical Technology awarded to Dr. Marvin Caruthers for developing technology that efficiently synthesizes DNA The inaugural Richard N. Caruthers was announced as the winner in June for his development, in 1981, of an efficient, automated technology for synthesizing DNA.
By Greta Friar, Whitehead Institute June 27, 2024 Images of a mouse brain show the effect of a technology called CHARM in turning off the expression of a gene in the brain. Like Cas9, ZFPs can serve as guide proteins to direct the tool to a target site in DNA. Credit: Neumann EN, Bertozzi TM, et al.
Predictive Models A “ sequence-to-function ” predictive model – an algorithm that determines a protein’s likely function solely by looking at the DNA sequence encoding it – is the natural successor to AlphaFold2. A predictive model for sequence-to-expression would raise the “hit” rates.
10 Reported cAMP as the second messenger signalling molecule conserved from bacteria to humans and modulates several biological processes, including proteinexpression, gene transcription, and cell development and differentiation. In this assay, active compounds are discovered under physiologically relevant conditions.
Predictive Models A “ sequence-to-function ” predictive model – an algorithm that determines a protein’s likely function solely by looking at the DNA sequence encoding it – is the natural successor to AlphaFold2. A predictive model for sequence-to-expression would raise the “hit” rates.
What are the key findings of Circio’s in vivo proof-of-concept for its circVec circular RNA platform technology compared to conventional mRNA-based expression with DNA vectors? Circular RNA (circRNA) has two major advantages versus mRNA in a vector-expression context. DNA vectors in mouse models?
This selectable marker enables the identification of cells which have taken up the GOIs and aids the initial selection of recombinant proteinexpressing cell lines. They work in the following way: the genes in an expression vector are flanked by inverted terminal repeat sequences (ITRs) to which a specific transposase enzyme can bind.
Data from standard DNA and RNA sequencing approaches were integrated with mass spectrometry-based proteomics and phosphoproteomic analyses to derive more complete molecular portraits of treatment-responsive versus treatment-resistant tumors. These data suggest a multi-omics predictor for chemotherapy response is within reach.
The analysis of circulating tumor DNA (ctDNA) in a liquid biopsy can also permit genotyping and help monitor the effectiveness of chemotherapy. Proteomics Proteomics involves studying the entire set of proteinsexpressed in a cell or tissue.
The success of PARP inhibitors in BRCA-mutant ovarian and breast cancers validates this approach, and similar strategies are being tested for other DNA repair enzymes. Indirectly Targeting "Undruggable" Proteins Small molecules can also target "undruggable" proteins indirectly by modulating the production of these proteins.
Fibroblast activation protein (FAP) is a cell-surface proteinexpressed at low levels in most normal adult tissues, but over-expressed in common cancers, particularly on cancer-associated fibroblasts that form the tumor stroma, which is essential for growth 1 ,2 ,3 ,4.
The researchers turned to CRISPR —a gene editing tool that uses “molecular scissors to cut DNA ,” according to Sanjana—to edit the regions identified by GWAS. The researchers then use CRISPR to target each of the regions of the genomes implicated by GWAS and conduct single-cell sequencing to evaluate gene and proteinexpression.
The current landscape of protein drug development is characterised by accelerated timelines where new drugs are approved in months rather than years. For example, advances in media and feeds have contributed to higher cell proteinexpression by optimising nutrient availability and reducing accumulation of waste products within a culture.
Several viral vectors are being used currently, in addition to non-viral vectors, such as oligonucleotides, naked DNA, and lipoplexes and polyplexes. They work by binding to specific sequences of nucleotides present within the mRNA structure and can induce mechanisms that either decrease, restore, or modify proteinexpression.
.” This technology could be used to design protein therapeutics that can bind to, and “shut down,” harmful or misfolded proteins in living cells. Thousands of transcription factors — proteins that bind DNA and control gene expression — were studied in human cells. Bergs A.C.F.
Genetic factors Mutations or alterations in tumour DNA (eg, EGFR mutations in lung cancer or HER2 amplification in breast cancer) are key targets of precision medicine. Molecular factors Molecular markers, such as proteinexpression and signalling pathway activation, also guide therapy choices.
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