DNA and Protein Production - Drug Discovery Digest

Gene editing extends lifespan in mouse model of prion disease

Broad Institute

JANUARY 14, 2025

The treatment, which uses base editing to make a single-letter change in DNA, reduced levels of the disease-causing prion protein in the brain by as much as 60 percent. In 2018, Liu, who works on the same floor as Minikel and Vallabh at Broad, approached them and proposed a collaboration.

Disease

Disease DNA Protein Production Treatment

Messenger RNAs with multiple “tails” could lead to more effective therapeutics

Broad Institute

MARCH 22, 2024

Messenger RNAs with multiple “tails” could lead to more effective therapeutics By Corie Lok March 22, 2024 Breadcrumb Home Messenger RNAs with multiple “tails” could lead to more effective therapeutics Scientists have engineered long lasting mRNAs that increased therapeutic protein production in cells and animals.

Engineering

Engineering Protein Production RNA Vaccine

Merkin Prize in Biomedical Technology awarded to F. William Studier for development of widely used protein- and RNA-production platform

Broad Institute

MAY 14, 2024

By 1984, he and Brookhaven colleague John Dunn successfully identified and cloned the protein within T7 that was responsible for rapidly copying T7 DNA into many corresponding strands of RNA — a critical step in the bacteriophage’s ability to infect E. coli genome and let the E. coli had no built-in way to shut it off.

RNA

RNA Production Molecular Biology Laboratories

Key considerations before commencing cell line development

Drug Target Review

JULY 5, 2023

After binding, the transposase enzyme will excise the flanked section of DNA (termed the transposon) and insert it into sites in the genome at specific target sequences. An example of the former includes a report in the journal Nature of a new tool that can increase the amount of DNA that can be inserted using CRISPR-Cas9 technology.

Engineering

Engineering DNA Laboratories Production

Traditional Meat Industry’s Beef With Alternative Protein Continues with the FAIR on Labels Act

FDA Law Blog: Biosimilars

MARCH 5, 2024

Gaulkin & Riëtte van Laack — As readers of this blog know, there is a lot of contention about the naming of alternative protein products (APPs), including both plant-based and cell-cultured alternatives for (traditional) animal products. By Sophia R.

Protein Production

Protein Production Production DNA Regulations

Proteogenomics reveals markers of chemotherapy resistance and outcome in triple negative breast cancer

Broad Institute

AUGUST 24, 2022

Data from standard DNA and RNA sequencing approaches were integrated with mass spectrometry-based proteomics and phosphoproteomic analyses to derive more complete molecular portraits of treatment-responsive versus treatment-resistant tumors. "The These data suggest a multi-omics predictor for chemotherapy response is within reach.

DNA

DNA Protein Expression Treatment Protein Production

Structural Biology

Sygnature Discovery

SEPTEMBER 12, 2024

Structures solved of many different protein classes, including membrane proteins, protein complexes and recently RNA. With capabilities across X-ray Crystallography Crystallisation/co-crystallisation screening of proteins, DNA, and RNA using in-house and commercial screens at various temperature.

Fragment Screening

Fragment Screening RNA Protein Production DNA

Cave Coronavirus in Wuhan Lab Seeded COVID – The Truth Has Always Been Out There, in the Genetics

PLOS: DNA Science

JUNE 13, 2024

Experiments at the Wuhan facility were done at the lowest two of the four standard biosafety levels, which were established at the dawn of recombinant DNA research in the 1970s. And a change to one specific part of the virus enabled it to locate and glom onto our cells, then slip inside and take over the protein production line.

Virus

Virus RNA DNA Science

Codon Digest: Discovering Antibiotics with Deep Learning

Codon

MAY 30, 2023

This week: A way to measure a transgene’s expression in the brain using ultrasound, a DNA sequencing method that uses 1000x less reagents, and base editors get even smaller. An engineered version of this protein can convert DNA bases with efficiencies up to 92%. so this Digest will be published more irregularly.

DNA

DNA RNA Engineering Vaccine

Codon Digest: Discovering Antibiotics with Deep Learning

Codon

MAY 30, 2023

This week: A way to measure a transgene’s expression in the brain using ultrasound, a DNA sequencing method that uses 1000x less reagents, and base editors get even smaller. An engineered version of this protein can convert DNA bases with efficiencies up to 92%. so this Digest will be published more irregularly.

DNA

DNA RNA Engineering Vaccine

Beyond Steel Tanks

Codon

MARCH 24, 2024

Credit: Universität Würzburg Archives His key insight hinged upon the fact that living cells are essentially bags of liquid filled with enzymes, or proteins that speed up chemical reactions. Water accounts for 70 percent of a bacterium by mass; the other 30 percent includes everything else: proteins, RNA, DNA, lipids, and so on.

Engineering

Engineering Vaccine Production RNA

Codon Digest: Bacteria Resist Every Virus

Codon

MARCH 20, 2023

Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers. Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast. Optogenetic closed-loop feedback control of the unfolded protein response optimizes protein production.

Virus

Virus Engineering RNA Small Molecule

A Look Back

Dark Matter Blog

JULY 16, 2021

As soon as I learned about DNA and RNA, I wanted to be a molecular biologist. Last stops at RNA My last roles in biotech were where my original passion began: DNA and RNA. 49 years ago, when I was 16 years old, I wanted to do exactly what I am doing now. The only subject in school that held my interest was biology.

Small Molecule

Small Molecule RNA Clinical Trials DNA

Article FDA Thank You Draft guidance on potency assays for CGT products garners extensive stakeholder input

Agency IQ

APRIL 12, 2024

Cell-based assays , while more complex to develop and use, offer another option for characterizing product activity at a slightly higher level, allowing sponsors to measure the further downstream effects of products (e.g., morphological cell changes or alterations in protein production or expression).

Production

Production FDA Therapies Biochemical Assays

An “Unsilencer” Drug May Treat Angelman Syndrome – Someday

PLOS: DNA Science

AUGUST 8, 2024

This part of chromosome 15 is especially unstable because highly repetitive DNA sequences bracket the genes associated with the symptoms. Repeats can cause slippage as DNA replicates. The researchers scrutinized a “chemogenetic library ,” which is a collection of chemical compounds that bind to specific DNA sequences.

Small Molecule

Small Molecule DNA Drugs Protein Production

Mutations in two gene pairs point to a promising drug target in 5 percent of adult cancers

Broad Institute

FEBRUARY 5, 2025

Another group of cells with high microsatellite instability mutations in short repeated DNA sequences, specifically in the TTC37 gene had the same sensitivity. PELO encodes a protein responsible for restarting ribosomes when they stall on defective mRNA sequences during protein production. But the cells with 9p21.3

Protein Production

Protein Production DNA Drugs RNA

The Codon Guide to Synthetic Biology

Codon

AUGUST 10, 2023

Technologies DNA Sequencing →DNA sequencing at 40: past, present and future , by Shendure J. Link DNA Cost and Productivity Data, aka "Carlson Curves" , by Carlson R. Link Next-Generation DNA Sequencing Methods , by Mardis E.R. Link DNA synthesis technologies to close the gene writing gap , by Hoose et al.

DNA

DNA Cell Biology Molecular Biology Engineering

The Codon Guide to Synthetic Biology

Codon

AUGUST 10, 2023

Technologies DNA Sequencing →DNA sequencing at 40: past, present and future , by Shendure J. Link DNA Cost and Productivity Data, aka "Carlson Curves" , by Carlson R. Link Next-Generation DNA Sequencing Methods , by Mardis E.R. Link DNA synthesis technologies to close the gene writing gap , by Hoose et al.

DNA

DNA Cell Biology Engineering Molecular Biology

Drug Discovery Digest

Gene editing extends lifespan in mouse model of prion disease

Messenger RNAs with multiple “tails” could lead to more effective therapeutics

Trending Sources

Merkin Prize in Biomedical Technology awarded to F. William Studier for development of widely used protein- and RNA-production platform

Key considerations before commencing cell line development

Traditional Meat Industry’s Beef With Alternative Protein Continues with the FAIR on Labels Act

Proteogenomics reveals markers of chemotherapy resistance and outcome in triple negative breast cancer

Structural Biology

Cave Coronavirus in Wuhan Lab Seeded COVID – The Truth Has Always Been Out There, in the Genetics

Codon Digest: Discovering Antibiotics with Deep Learning

Codon Digest: Discovering Antibiotics with Deep Learning

Beyond Steel Tanks

Codon Digest: Bacteria Resist Every Virus

A Look Back

Article FDA Thank You Draft guidance on potency assays for CGT products garners extensive stakeholder input

An “Unsilencer” Drug May Treat Angelman Syndrome – Someday

Mutations in two gene pairs point to a promising drug target in 5 percent of adult cancers

The Codon Guide to Synthetic Biology

The Codon Guide to Synthetic Biology

Stay Connected