Broad Institute

MARCH 22, 2024

Messenger RNAs with multiple “tails” could lead to more effective therapeutics By Corie Lok March 22, 2024 Breadcrumb Home Messenger RNAs with multiple “tails” could lead to more effective therapeutics Scientists have engineered long lasting mRNAs that increased therapeutic protein production in cells and animals.

Engineering 140

Engineering Protein Production RNA Vaccine 140

Codon

MARCH 20, 2023

Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers. Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast. Optogenetic closed-loop feedback control of the unfolded protein response optimizes protein production.

Virus 52

Virus Engineering RNA Small Molecule 52

Broad Institute

MAY 14, 2024

His T7 expression technology can be used to make large quantities of nearly any RNA or protein and has been for decades, and continues to be, a mainstay of biomedical research and pharmaceutical production. coli polymerase produce the corresponding RNA at the same time as the bacteria produced its own RNA and proteins.

RNA 84

RNA Production Molecular Biology Laboratories 84

Codon

MARCH 24, 2024

Credit: Universität Würzburg Archives His key insight hinged upon the fact that living cells are essentially bags of liquid filled with enzymes, or proteins that speed up chemical reactions. Water accounts for 70 percent of a bacterium by mass; the other 30 percent includes everything else: proteins, RNA, DNA, lipids, and so on.

Engineering 86

Engineering Vaccine Production RNA 86

Codon

MAY 30, 2023

This week: A way to measure a transgene’s expression in the brain using ultrasound, a DNA sequencing method that uses 1000x less reagents, and base editors get even smaller. An engineered version of this protein can convert DNA bases with efficiencies up to 92%. so this Digest will be published more irregularly.

DNA 52

DNA RNA Engineering Vaccine 52

Codon

MAY 30, 2023

This week: A way to measure a transgene’s expression in the brain using ultrasound, a DNA sequencing method that uses 1000x less reagents, and base editors get even smaller. An engineered version of this protein can convert DNA bases with efficiencies up to 92%. so this Digest will be published more irregularly.

DNA 52

DNA RNA Engineering Vaccine 52

Codon

AUGUST 10, 2023

Technologies DNA Sequencing →DNA sequencing at 40: past, present and future , by Shendure J. Link DNA Cost and Productivity Data, aka "Carlson Curves" , by Carlson R. Link Next-Generation DNA Sequencing Methods , by Mardis E.R. Link DNA synthesis technologies to close the gene writing gap , by Hoose et al.

DNA 52

DNA Cell Biology Engineering Molecular Biology 52