Fierce BioTech

OCTOBER 31, 2024

The tools developed function entirely post-translationally, enabling synergistic strategies with engineerable regulation across multiple levels of the central dogma. The tools developed function entirely post-translationally, enabling synergistic strategies with engineerable regulation across multiple levels of the central dogma.

DNA 64

DNA Regulations 64

Broad Institute

JUNE 28, 2023

Merkin Prize in Biomedical Technology for developing an efficient, automated technology for synthesizing DNA. The chemical reactions that he discovered in the early 1980s to accurately and quickly assemble nucleotides into strands of DNA provided an essential element in the development of modern molecular medicine.

DNA 98

DNA RNA Science Therapies 98

addgene Blog

JUNE 20, 2024

As described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or may not be synchronized with the replication of the host cell's chromosomal DNA. All plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent.

Regulations 81

Regulations DNA 81

Covalent Modifiers

DECEMBER 11, 2023

Using covalent scout fragments, KB02 and KB05, we identified ligandable cysteines on proteins involved in spindle assembly, DNA repair, and transcriptional regulation, such as Cys101 of histone acetyltransferase 1 (HAT1). TurboID (His-TID) construct, with chemoproteomics.

Small Molecule 162

Small Molecule DNA Regulations Disease 162

Covalent Modifiers

MAY 25, 2024

3c12240 Caspases are a highly conserved family of cysteine-aspartyl proteases known for their essential roles in regulating apoptosis, inflammation, cell differentiation, and proliferation. Backus Journal of the American Chemical Society 2024 DOI: 10.1021/jacs.3c12240

Small Molecule 162

Small Molecule DNA Virus Regulations 162

SCIENMAG: Medicine & Health

JANUARY 16, 2024

The blueprint for human life lies within the DNA in the nucleus of each of our cells. DNA condensation is not random. In human cells, around six and a half feet of this genetic material must be condensed to fit inside the nucleus. To function properly, the genetic material is highly organized into loop structures […]

Regulations 84

Regulations DNA 84

Broad Institute

AUGUST 7, 2023

A surprising new role for a major immune regulator By Tom Ulrich August 7, 2023 Breadcrumb Home A surprising new role for a major immune regulator By Anne Trafton, MIT News August 7, 2023 Credit: RCSB Protein Data Bank A model of the human STING protein.

Regulations 91

Regulations Clinical Trials Engineering Science 91

Drug Target Review

SEPTEMBER 8, 2023

This receptor, referred to as retinoic acid receptor alpha (RARα), is recognised for its regulation of gene expression within the nucleus. The cell’s nucleus, containing compacted DNA, is centrally positioned within the cell.

Regulations 98

Regulations Research DNA Immune Response 98

Drug Target Review

JULY 7, 2023

The reasons for this are multifaceted, including concerns over the safety of directly altering DNA sequences and subsequent regulatory restrictions that have arisen as a result. The epigenome (meaning ‘above the genome’) is a system of reversible marks regulating how the DNA is read, translated and used.

DNA 106

DNA Engineering Therapies Regulations 106

ASPET

SEPTEMBER 16, 2024

Standard of care GBM therapies include radiation and cytotoxic chemotherapy that lead to DNA damage. Subsequent activation of DNA damage response (DDR) pathways can induce resistance. Elimusertib, a novel ATR kinase inhibitor, can prevent repair of damaged DNA, increasing efficacy of DNA damaging cytotoxic therapies.

Pharmacogenetics 100

Pharmacogenetics DNA Treatment Pharmacokinetics 100

Broad Institute

OCTOBER 23, 2024

That is in part because of the ongoing challenge of understanding the DNA switches, called cis-regulatory elements (CREs), that control the expression and repression of genes. CREs themselves are not part of genes, but are separate, regulatory DNA sequences – often located near the genes they control. In a paper published in Oct.

Research 136

Research DNA Laboratories Molecular Biology 136

ASPET

MARCH 20, 2024

DNA topoisomerase IIβ (TOP2β/180; 180 kDa) is a nuclear enzyme that regulates DNA topology by generation of short-lived DNA double-strand breaks primarily during transcription. TOP2β/180 can be a target for DNA damage-stabilizing anticancer drugs, whose efficacy is often limited by chemoresistance.

DNA 100

DNA Laboratories Therapies Regulations 100

Broad Institute

JUNE 10, 2024

The new method uses a combination of prime editing, which can directly make a wide range of edits up to about 100 or 200 base pairs, and newly developed recombinase enzymes that efficiently insert large pieces of DNA thousands of base pairs in length at specific sites in the genome.

DNA 144

DNA Engineering Therapies Disease 144

Drug Target Review

NOVEMBER 3, 2023

They identified ZNF800 as a key regulator of the differentiation of a specific gut cell type, the enteroendocrine cells. These hormones regulate digestive processes, like nutrient absorption, appetite, and glucose metabolism. The study was published in Science.

Research 111

Research Regulations DNA Government 111

SCIENMAG: Medicine & Health

AUGUST 3, 2023

STING is primarily on the lookout for DNA, which can indicate either a foreign invader such as a virus or damage to the […] CAMBRIDGE, MA — A signaling protein known as STING is a critical player in the human immune system, detecting signs of danger within cells and then activating a variety of defense mechanisms.

Regulations 64

Regulations DNA Virus 64

Chemical Biology and Drug Design

SEPTEMBER 28, 2023

Abstract Proliferating cell nuclear antigen (PCNA) is a homo-trimeric protein complex that clamps around DNA to tether DNA polymerases to the template during replication and serves as a hub for many other interacting proteins. This is the proof-of concept for a novel T. brucei

Small Molecule 100

Small Molecule DNA Regulations Research 100

NIH Director's Blog: Drug Development

MARCH 11, 2014

Credit: Jane Ades, National Human Genome Research Institute, NIH Type 2 diabetes (T2D) tends to run in families, and over the last five years the application of genomic technologies has led to discovery of more than 60 specific DNA variants that contribute to risk. Nat Rev Drug Discov. 2013 Aug;12(8):581-94. [3]

DNA 52

DNA Drugs Disease Research Laboratories 52

Drug Target Review

NOVEMBER 13, 2023

The instructions to form a cell are encoded in DNA strands, wrapped in proteins and RNA, and wound together into a 3D structure called chromatin. Another finding was that regulatory T cells had distinct chromatin architecture features called DNA loops. This indicated other Foxp3 protein-containing complexes may be involved.

Government 105

Government DNA RNA Regulations 105

KIF1A

JANUARY 13, 2024

This process is regulated so we end up with the right number of neurons, which we can imagine with some simplified math: If you start with one progenitor and need eight neurons, it isn’t efficient for that progenitor to make a single neuron eight times.

DNA 105

DNA Regulations Drug Development Disease 105

Chemical Biology and Drug Design

NOVEMBER 23, 2023

Multiple investigations prove that it is regulated genetically, and epigenetically, and is affected by environmental factors. The molecular and neural pathways linked to the regulation of schizophrenia have been extensively studied.

Therapies 100

Therapies DNA Regulations Disease 100

Broad Institute

OCTOBER 4, 2023

Related links Merkin Prize Inaugural Merkin Prize in Biomedical Technology awarded to Dr. Marvin Caruthers for developing technology that efficiently synthesizes DNA The inaugural Richard N. Caruthers was announced as the winner in June for his development, in 1981, of an efficient, automated technology for synthesizing DNA.

DNA 52

DNA RNA Science Protein Expression 52

ASPET

JUNE 10, 2024

Radiation therapy, a standard treatment option for many cancer patients, induces DNA double strand breaks (DSBs), leading to cell death. Ataxia telangiectasia mutated (ATM) kinase is a key regulator of DSB repair, and ATM inhibitors are being explored as radiosensitizers for various tumors, including primary and metastatic brain tumors.

Pharmacokinetics 100

Pharmacokinetics Pharmacogenetics Treatment DNA 100

Broad Institute

AUGUST 14, 2023

In three new studies, researchers including a team from the Broad Institute of MIT and Harvard have harmonized and analyzed data on proteins, DNA, RNA, as well as clinical data from more than 1,000 patients across nearly a dozen different cancer types. They then characterized 33 molecular signatures to group biologically similar tumors.

DNA 98

DNA RNA Regulations Treatment 98

Conversations in Drug Development Trends

NOVEMBER 12, 2024

For example, once considered incurable and terminal, patients with sickle cell disease may reach new summits in their lives with gene editing technologies such as CRISPR to repair affected DNA and, in some cases, functionally cure the condition. Operationalizing these trials requires proactive and flawless management at every stage.

Therapies 72

Therapies Clinical Supply Clinical Trials Trials 72

Chemical Biology and Drug Design

DECEMBER 12, 2024

Biochemical kits was utilized for checking the ATP, mitochondrial DNA, MDA, GSH, and Fe 2+ levels in the Huh7 cells, and western blot for measuring the ferroptosis and AMPK/mTOR related-protein expression levels. SchA also induced apoptosis in a dose-dependent manner.

Protein Expression 100

Protein Expression DNA Regulations Research 100

DrugBaron

JANUARY 8, 2021

Sarepta is no stranger to controversy, following the decision of the US regulator to approve the company’s earlier product, eteplirsen, which induces exon-skipping to improve expression of dystrophin protein in individuals who carry the mutant gene, as Exondys 51 back in 2016.

DNA 100

DNA Therapies Disease Production 100

Codon

FEBRUARY 13, 2024

Regulators traditionally want to see a single, stable, well-characterized drug before giving the green light for it to be tested in a clinical trial, not dozens of different viruses; let alone ones that are best found in unappealing places like sewage , hospital waste, or bird poop.

Therapies 124

Therapies Virus DNA Clinical Trials 124

ASPET

JANUARY 22, 2024

The NOD-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome is a central regulator of innate immunity, essential for processing and release of interleukin-1β and pyroptotic cell death. Mice also contain plasma esterase (Ces1c) activity which is absent in humans.

Pharmacokinetics 100

Pharmacokinetics DNA Engineering International 100

Drug Target Review

JULY 24, 2023

Researchers from Tokyo Tech’s World Research Hub Initiative conducted a study to investigate the regulation of gene expression in the apicoplast of the malaria parasite Plasmodium falciparum. How is melatonin, the circadian signalling hormone, related to the regulation of gene expression in the apicoplast?

RNA 98

RNA Regulations Bioinformatics Laboratories 98

Advarra

JUNE 12, 2023

Gene therapy research is exciting and full of promise, but because of the risks involved, it’s also highly regulated, requiring an institutional biosafety committee (IBC) to provide additional oversight and risk assessment. What do the Regulations Say about IBC Review?

Engineering 98

Engineering Therapies Regulations Research 98

Drug Target Review

OCTOBER 12, 2023

Although non-coding variants have been increasingly appreciated for their essential role in controlling gene expression across cell types and tissues, scientists find it difficult to match them to the genes they regulate, the effects on AD-related functions and thus the individual risk of contracting AD.

Regulations 103

Regulations DNA Disease Research 103

Drug Target Review

AUGUST 9, 2023

The specific proteins found in these aggregates can vary, but approximately 95 percent of ALS patients and 50 percent of FTD patients develop aggregates containing the TDP-43 protein (short for tar DNA binding protein 43). 14 TDP-43 is a DNA and RNA binding protein that regulates the expression and splicing of several target transcripts.

RNA 98

RNA Regulations Disease DNA 98

Codon

AUGUST 26, 2024

DNA delivered to the nose or lungs can express an encoded protein for months to over a year in mice if the vector—the “backbone” of DNA-based medicines controlling the level and timing of expression of the encoded protein—is specifically engineered to be long-lasting.

RNA 94

RNA Virus DNA Vaccine 94

Codon

JUNE 25, 2024

However, they plod along as they clone plasmids—the loops of DNA that biologists use to manipulate and study organisms—because propagating them relies, in part, on the pace at which cells grow and divide. Most medicines, including insulin and semaglutide (the weight loss drug), are made using DNA cloning. However, E.

DNA 75

DNA RNA Laboratories Research 75

BMG Labtech

DECEMBER 20, 2024

Whether it is determining concentrations of molecules like proteins or DNA, looking at enzyme kinetics for crucial reactions, or measuring something as fundamental as cell growth, you will find references to absorbance or optical density measurements. Optical density is also widely used for many scientific applications.

DNA 52

DNA Science Laboratories RNA 52

Broad Institute

FEBRUARY 27, 2024

Past Speakers Speaker Topic Jeffery Kelly Pharmacological Adaptation of Proteostasis to Ameliorate Aging-associated Degenerative Diseases Chuan He RNA modification in gene expression regulation Richard Young Biomolecular condensates: new insights into regulation and dysregulation of cellular compartments David Liu Base Editing and Prime Editing: (..)

RNA 52

RNA DNA Regulations Disease 52

Fierce BioTech

JUNE 15, 2023

Collecting DNA methylation data by WGBS offers valuable insights into disease origin, predisposition, and treatment response, potentially guiding therapeutic strategies. Collecting DNA methylation data by WGBS offers valuable insights into disease origin, predisposition, and treatment response, potentially guiding therapeutic strategies.

DNA 52

DNA Clinical Research Regulations Disease 52