Broad Institute

JANUARY 8, 2024

Williams January 8, 2024 Credit: Susanna Hamilton, Broad Communications Researchers have developed virus-like particles that can deliver gene-editing cargo to cells, including those in the mouse brain. Prime editing, described in 2019 by Liu’s group, can make longer and more diverse types of DNA changes than other types of editing.

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Engineering Research Virus DNA 144

Covalent Modifiers

MAY 25, 2024

By combining both gel-based activity-based protein profiling (ABPP) and a tobacco etch virus (TEV) protease activation assay, we then identify covalent lead compounds that react preferentially with this cysteine and afford a complete blockade of caspase-2 activity.

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Small Molecule DNA Virus Regulations 162

Broad Institute

AUGUST 31, 2023

Their new editors are more efficient and specialized than previous versions, and are able to modify DNA in cultured cells and in animals that have been difficult to edit, including in immune system cells and inside the brain. In the new work, they focused on improving the heart of the prime editing system—the reverse transcriptase.

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DNA RNA Engineering Laboratories 145

SCIENMAG: Medicine & Health

JANUARY 24, 2024

Viral genetic material was integrated into the genome of the first multi-cellular beings and is still in our DNA today. All animals have evolved thanks to the fact that certain viruses infected primitive organisms hundreds of millions of years ago.

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Virus DNA Science Research 69

PLOS: DNA Science

MAY 16, 2024

Many of the links below are to some of the 100+ DNA Science posts that I wrote during the pandemic. A Novel Virus Appears In retrospect, everything unfolded with astonishing speed. Whenever and wherever the virus emerged, CDC officially alerted clinicians to the new, deadly respiratory illness on January 8, 2020.

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Virus DNA RNA Vaccine 98

Drug Target Review

JUNE 28, 2023

When faced with a viral threat, bacterial cells developed an immune response by capturing and copying DNA fragments of viruses. This allowed bacteria to recognise subsequent attacks and cleave the viral DNA to stop the viral infection. It was also discovered that the Cas enzyme was responsible for DNA cleavage.

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Engineering DNA Immune Response RNA 98

Codon

AUGUST 26, 2024

When COVID-19 emerged in 2019, by contrast, mRNA vaccines developed by Pfizer and Moderna took just 326 days from the initial sequencing of the virus to gaining approval for emergency use. These drugs would be ideal tools to bridge the wait for a vaccine against a quickly-spreading virus.

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RNA Virus DNA Vaccine 100

PLOS: DNA Science

JANUARY 25, 2024

We can learn about life, past and present, anywhere we find DNA and determine its sequence. DNA Science has described intriguing sources of environmental DNA, aka eDNA: DNA in Strange Places: Hippo Poop, Zoo Air, and Cave Dirt and A Glimpse of the Ocean’s Twilight Zone Through Environmental DNA.

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Disease DNA Science Virus 98

Codon

AUGUST 27, 2024

Allison Berke makes the case for real-time DNA sequencing and AI tools to detect pathogens before they spread widely. Reading DNA The first step in detecting a novel pathogen is recognizing it as an anomaly amidst a noisy background of other material. After copying the DNA to form a big pool, each piece is sequenced.

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DNA Laboratories Hospitals Virus 100

PLOS: DNA Science

JULY 27, 2023

Genomics applies to all species, revealing evolution in action, because we all use the same genetic code – that is, the correspondence between DNA sequences and the amino acid sequences of proteins. Cats and Bird Flu Comparing DNA sequences is a little like linguistic research that connects languages. emerged in late 2021.

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Disease DNA Virus RNA 95

Drug Target Review

SEPTEMBER 28, 2023

After some time in that role and launching several products, I received a call from Bill Banyai and Bill Peck, or ‘The Bills’ as we call them, who were building a company around technology that creates DNA by ‘writing’ it on a silicon chip. These are synthetic, not the live virus, so do not pose a risk of infection.

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DNA Organic Chemistry Science Virus 116

Broad Institute

JUNE 10, 2024

The new method uses a combination of prime editing, which can directly make a wide range of edits up to about 100 or 200 base pairs, and newly developed recombinase enzymes that efficiently insert large pieces of DNA thousands of base pairs in length at specific sites in the genome.

DNA 144

DNA Engineering Therapies Disease 144

Drug Target Review

JANUARY 26, 2024

These ACE2-coated particles could be tested as an anti-viral therapeutic, and as sensors for detecting virus particles. To find a DNA fragment that contained the intact ACE2 gene, complete with its embedded regulatory information, they searched libraries of cloned DNA fragments generated as part of the Human Genome Project.

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Production DNA Virus Protein Expression 114

Codon

FEBRUARY 13, 2024

doi: 10.2210/rcsb_pdb/goodsell-gallery-048 The Virus that Cures It’s been over 25 years since the science magazine Discover first ran an extraordinary article about how a long-forgotten medical treatment, used in the former Soviet country of Georgia, could save us from the growing threat of untreatable, drug-resistant infections.

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Therapies Virus DNA Clinical Trials 129

PLOS: DNA Science

APRIL 20, 2023

DNA is a sleek double helix, with “rungs” consisting of a purine base paired with a smaller pyrimidine base: adenine (A) with thymine (T) and guanine (G) with cytosine (C). . ” DNA encodes amino acid sequences comprising proteins, which impart traits. Watson and F.

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DNA Virus Science RNA 52

SCIENMAG: Medicine & Health

JUNE 16, 2023

As a part of its life cycle, the human immunodeficiency virus-1 (HIV) inserts a copy of its DNA into human immune cells. Some of these newly infected immune cells can then transition into a dormant, latent state for a long period of time, which is referred to as HIV latency. Credit: UNC Division of Infectious […]

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DNA Research Virus 82

NIH Director's Blog: Drug Discovery

MARCH 8, 2016

Instead of the black, printed stripes of the Universal Product Codes (UPCs) that we see on everything from package deliveries to clothing tags, they used short, unique snippets of DNA to label cells. DNA barcoding has already empowered single-cell analysis, including for nerve cells in the brain. PRISM consists of two key components.

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DNA Drugs Small Molecule FDA Approval 52

PLOS: DNA Science

FEBRUARY 8, 2024

DNA Science recently covered perfumes resurrected from extinct plants, but using older and less precise recombinant DNA technology. appeared first on DNA Science. But will it, can it, improve upon nature? Several species of australopithecines, for example, lived in Africa 2 to 4 million years ago. CRISPR Hubris?

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Immune Response DNA Engineering Virus 98

Broad Institute

DECEMBER 13, 2023

They transferred 101 serial sections, spanning the volume of a single mouse brain, onto arrays of beads covered in unique DNA barcodes, which bound to the mRNA transcripts in the brain tissue. In their study, the researchers focused on a variant of an engineered recombinant adeno-associated virus (rAAV) known as PHP.eB.

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DNA Virus Research Engineering 140

SCIENMAG: Medicine & Health

JULY 11, 2023

The immune system is one of the most complex parts of our body. It keeps us healthy by getting rid of parasites, viruses or bacteria, and by destroying damaged or cancer cells. One of its most intriguing abilities is its memory: upon first contact with a foreign component (called “antigens” in scientific jargon) our adaptive […]

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DNA Virus 60

SCIENMAG: Medicine & Health

AUGUST 7, 2023

Prolonged exposure to UVR is associated with damage to DNA in skin cells, […] Skin, the largest organ of the human body, is home to a vast array of bacteria, fungi, and viruses – microorganisms that compose the skin microbiota.

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DNA Virus 75

Drug Target Review

JULY 5, 2023

Here, the specificity of the protein is built into the gene-editing protein itself and, unlike earlier generation gene-editing systems, this system is not designed to create double-strand breaks in genomic DNA, but rather is designed to create nicks or single-strand breaks in genomic DNA.

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Therapies DNA Engineering Virus 103

Broad Institute

JUNE 27, 2024

Unlike gene editing, this “epigenetic” editing does not modify the underlying DNA sequence, but it should switch the gene off permanently, which means that this could be a one-time treatment. They used an engineered adeno-associated virus (AAV) that crosses the blood-brain barrier after intravenous administration.

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Disease Therapies Clinical Trials Engineering 142

PLOS: DNA Science

AUGUST 22, 2024

.” The explanation continues, and here’s where it ceases to make sense, to anyone who knows anything about DNA, genes, and genomes. Then viruses in the poop enter leaf cells, doing what viruses do and commandeering the cellular DNA replication machinery to make more of themselves. BUT WAIT!!!

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DNA Virus Organic Chemistry Science 71

Codon

MARCH 20, 2023

Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers. CRISPR-induced DNA reorganization for multiplexed nucleic acid detection. Combining fusion of cells with CRISPR-Cas9 editing for the cloning of large DNA fragments or complete bacterial genomes in yeast.

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Virus Engineering RNA Small Molecule 52

Codon

JUNE 11, 2024

And unlike traditional DNA sequencers, which parse genetic material by breaking it up into fragments and interpreting it chunk-by-chunk, a nanopore device unspools a long strand of DNA and reads it all at once. A scientist can isolate DNA and load up a flow cell in fifteen minutes. Nanopore devices work incredibly fast.

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DNA Engineering RNA Small Molecule 85

Drug Target Review

JANUARY 15, 2024

Each T cell expresses a TCR specific for an HLA‑presented peptide signal, and when that signal is detected a T cell expressing the relevant TCR can launch a complex array of immune effector responses that can suppress or destroy tumours or virus-infected cells.

Engineering 110

Engineering DNA Virus Immune Response 110

Codon

SEPTEMBER 29, 2024

mRNA was the intermediate stage between DNA and protein, a dynamic entity that shifted depending on the second-to-second needs of the cell, able to point out if a cell was cancerous or stressed, what kind of cell it was, and so on. Collections of mRNA have been understood as a proxy for cell states for decades.

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Engineering Virus DNA Therapies 141

Codon

DECEMBER 12, 2024

Some scientists even think it had a virus defense system; “researchers say LUCA likely housed 19 CRISPR genes, which bacteria use to slice up viral threats,” reports Quanta Magazine. DNA and RNA molecules are also built from exclusively right-handed nucleic acids.

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DNA RNA Vaccine Virus 104

SCIENMAG: Medicine & Health

AUGUST 3, 2023

STING is primarily on the lookout for DNA, which can indicate either a foreign invader such as a virus or damage to the […] CAMBRIDGE, MA — A signaling protein known as STING is a critical player in the human immune system, detecting signs of danger within cells and then activating a variety of defense mechanisms.

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Regulations DNA Virus 64

PLOS: DNA Science

SEPTEMBER 7, 2023

As genetics morphed into genomics, artificial intelligence stepped in, layering the combinatorial information of comparative genomics onto DNA sequences. Train an algorithm on the DNA sequences of a known disease-causing gene, then search for identical or highly similar sequences in cells from other individuals to assist diagnosis.

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DNA Science Therapies Licensing 98

Drug Target Review

OCTOBER 26, 2023

Genome engineering and gene therapies that manipulate DNA sequences in cells have driven a biotechnological revolution over the past decade. 1 Adeno-associated virus (AAV) vectors are the leading platform for in vivo gene delivery for the treatment of various human diseases. Engineering adeno-associated virus vectors for gene therapy.

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Engineering Virus Therapies Immune Response 105

PLOS: DNA Science

JUNE 13, 2024

The virus SARS-CoV-2 emerged in Wuhan, home of a premiere research institute that focuses on viruses. Experiments at the Wuhan facility were done at the lowest two of the four standard biosafety levels, which were established at the dawn of recombinant DNA research in the 1970s. An altered virus could have escaped. Coincidence?

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Virus RNA DNA Science 52

Drug Target Review

DECEMBER 28, 2023

During the process of transformation from a normal cell into a cancer cell, a cell acquires a series of changes, or mutations, in its DNA. But DNA mutations can also result in changes to the proteins that are displayed on the surface of the cancer cell. Our immune system helps us to fight off infections and cancer.

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Bioinformatics Immune Response DNA Vaccine 105

Codon

JUNE 25, 2024

However, they plod along as they clone plasmids—the loops of DNA that biologists use to manipulate and study organisms—because propagating them relies, in part, on the pace at which cells grow and divide. Most medicines, including insulin and semaglutide (the weight loss drug), are made using DNA cloning. However, E.

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DNA RNA Laboratories Research 80

addgene Blog

MAY 30, 2023

Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. This post was contributed by guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik.

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Virus Packaging DNA Laboratories 58

Drug Target Review

SEPTEMBER 4, 2023

Infectious agent includes bacteria, virus, fungi, and parasites, and they are capable of causing disease in a living organism. I had earlier proposed the use of Palmatine and Silver Nitrate in the efficacy against the RNA structure of the COVID-19 virus. We discovered it is highly effective and environmentally friendly.

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Drugs RNA DNA Disease 111