addgene Blog

JULY 30, 2024

Viral vectors are viruses engineered to be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding their wild counterparts.

Engineering 97

Engineering Virus 97

SCIENMAG: Medicine & Health

AUGUST 16, 2023

(Philadelphia, PA) – A single injection of a novel CRISPR gene-editing treatment safely and efficiently removes SIV – a virus related to the AIDS-causing agent HIV – from the genomes of non-human primates, scientists at the Lewis Katz School of Medicine at Temple University now report.

Virus 77

Virus Treatment Engineering 77

Broad Institute

JULY 19, 2023

By Allessandra DiCorato July 19, 2023 Credit: Kevin Middleton, Broad Communications A three-dimensional model of adeno-associated viruses (AAVs), which scientists have engineered to package and deliver gene therapies to cells in the body. Qin Huang, a senior research scientist in Deverman’s group, is the first author on the study.

Therapies 98

Therapies Research Engineering Virus 98

Broad Institute

FEBRUARY 27, 2025

Nature is pretty incredible, said Zhang, who is also an investigator at the McGovern Institute and the Howard Hughes Medical Institute, a core member of the Broad Institute, a professor of brain and cognitive sciences and biological engineering at MIT, and co-director of the K. Lisa Yang and Hock E. These were the TIGR-Tas systems.

RNA 66

RNA Therapies DNA Virus 66

The Pharma Data

MAY 19, 2023

HIGH SCHOOL SCIENTISTS AND ENGINEERS WIN NEARLY $9 MILLION AT THE REGENERON INTERNATIONAL SCIENCE AND ENGINEERING FAIR 2023 Regeneron Pharmaceuticals, Inc. This allowed him to identify the mutations in the virus that likely made it more infectious as well as other mutations that could make it resistant to antibiotics.

Engineering 52

Engineering International Science Virus 52

Broad Institute

AUGUST 31, 2023

Now researchers at the Broad Institute of MIT and Harvard have used cutting-edge continuous laboratory evolution and engineering methods to develop improved versions of the gene-editing tool. Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Paper cited: Doman JL, Pandey S, et al.

DNA 145

DNA RNA Engineering Laboratories 145

The Pharma Data

MARCH 23, 2022

To treat Ebola virus infections, researchers are taking a close look at a key piece of the virus: polymerase. Polymerase is a viral protein that directs how Ebola virus replicates its genome as it infects new hosts. Drugs that target polymerase could potentially treat Ebola virus infections and save lives.

Virus 52

Virus Small Molecule Protein Production Research 52

Drug Target Review

JULY 5, 2023

iPSC-derived lymphocytes, eg, T cells and natural killer (NK) cells, engineered to express targeting molecules such as chimeric antigen receptors (CARs) have shown clinical promise to treat haematological malignancies. We believe this lays the groundwork for engineering these kinds of myeloid cells to potentially target any cancer antigen.

Therapies 104

Therapies DNA Engineering Virus 104

Codon

OCTOBER 13, 2024

Genetic engineering in the medicine cabinet is one thing; allowing it at the dinner table is another. Through genetic engineering, researchers have developed Golden Rice which can help address the problem of vitamin A deficiency , cotton that produces its own insecticide , virus-resistant papayas , and more. At the dining table.

Engineering 101

Engineering Virus Production DNA 101

PLOS: DNA Science

FEBRUARY 8, 2024

Already, engineered probiotics have been used to treat metabolic disorders like inflammatory bowel disease and obesity, single-gene conditions like PKU, and bacterial infections common in people who have cystic fibrosis. But will it, can it, improve upon nature? Probiotics are an enticing target. Sometimes, nature may be better.

Immune Response 97

Immune Response DNA Engineering Virus 97

Drug Target Review

AUGUST 11, 2023

In a new development, a recent paper published in Biology Methods & Protocols by Oxford University Press has highlighted a promising avenue for enhancing vaccine efficacy against infectious pathogens like the COVID-19 virus. The role of extracellular vesicles in COVID-19 virus infection [Internet]. Available from: [link]

Vaccine 98

Vaccine Immune Response Bioinformatics Virus 98

Broad Institute

AUGUST 7, 2024

Existing methods of engineering one of the most commonly used gene-delivery vehicles, adeno-associated viruses (AAV), are often slow and inefficient. Now, researchers at the Broad Institute of MIT and Harvard have developed a machine-learning approach that promises to speed up AAV engineering for gene therapy. Nature Communications.

Therapies 84

Therapies Research Engineering Packaging 84

Broad Institute

DECEMBER 13, 2023

The other study was led by senior authors Xiao Wang , a Broad core institute member, a Merkin Institute Fellow, and an assistant professor of chemistry at MIT, and Jia Liu, an assistant professor of engineering at Harvard University. Our atlas represents the largest spatial single-cell dataset our lab has analyzed to date,” said Wang.

DNA 140

DNA Virus Research Engineering 140

The Pharma Data

AUGUST 15, 2021

Polymersomes trigger robust T cell immunity, and the researchers wondered if they could further improve the antibody response by engineering the nanoparticles to mimic viruses by displaying multiple copies of the RBD on their surfaces. So the team made polymersomes that were similar in size to SARS-CoV-2 and decorated them with many RBDs.

Vaccine 52

Vaccine Virus Immune Response Clinical Trials 52

The Pharma Data

DECEMBER 22, 2020

SAITAMA, Japan–( BUSINESS WIRE )– After a capital increase in the initial closing of its series A round, with investments by Mitsubishi UFJ Capital and Real Tech Fund , Epsilon Molecular Engineering Inc. The bio industry has progressed to a point where it deals with proteins from an engineering perspective. Source link.

Engineering 52

Engineering Pharmaceuticals Drugs Protein Production 52

Drug Target Review

APRIL 5, 2024

She brings nearly two decades of experience in the pharmaceutical industry, and deep expertise in early drug discovery, antibody engineering (bispecifics), stable cell line development, and project management. The first concern is microbial contamination. CDMOs must routinely test throughout CLD to ensure cell cultures are free of microbes.

Protein Production 96

Protein Production Protein Expression Bioinformatics Production 96

NIH Director's Blog: Drug Discovery

NOVEMBER 5, 2020

They have been specially engineered to look and, in some ways, act like the coronavirus while helping to solve a real challenge for many labs that would like to study SARS-CoV-2. While the quantum dots may bind and enter human cells just like the virus, they can’t cause an infection.

Research Laboratories 85

Research Laboratories Laboratories Science Drugs 85

NIOSH Science Blog: Drugs

FEBRUARY 3, 2023

Air filtration units were widely used during large wildfire outbreaks in the western United States,[1] and their use surged nationwide during the COVID-19 pandemic as a way to reduce exposure to aerosols containing the SARS-CoV-2 virus.

Virus 97

Virus Production Engineering Disease 97

Broad Institute

JULY 11, 2024

Targeted drug treatment leads tumor cells to imitate viral infection By Ari Navetta July 11, 2024 Breadcrumb Home Targeted drug treatment leads tumor cells to imitate viral infection Exploiting "viral mimicry," mIDH1 inhibitors trick tumors into thinking they are infected with a virus.

Treatment 72

Treatment Immune Response Drugs Virus 72

Advarra

AUGUST 31, 2022

These injuries are often associated with occupational exposure to human bloodborne pathogens such as hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). A loaded syringe can be safely capped using either: A safety engineered syringe. Safety-engineered Syringes. Recapping tools.

Production 52

Production Engineering Pharmacy Virus 52

Advarra

AUGUST 8, 2023

Gene therapy involves the transfer of engineered genetic materials to human research subjects. Viral infection involves the transfer of the virus’ genetic material to host cells, making viruses ideal tools for gene transfer—once the genes responsible for viral replication and disease are removed.

Therapies 52

Therapies Research Engineering Clinical Research 52

Broad Institute

SEPTEMBER 17, 2024

Eventually Martin, always looking to grow her skillset, became the manager of that team, learning to guide engineers in their work. I provide technical guidance, empowering our engineers to do their best work. When you want to model how proteins interact with each other or with a virus, you can model that using GPUs.

Research 93

Research Science Engineering Virus 93

Broad Institute

SEPTEMBER 13, 2023

Some are focused on viruses called adeno-associated viruses (AAVs), which have been engineered to act only as a one-time delivery vehicle and not cause disease. Broad researchers are further engineering AAVs to efficiently deliver gene-based therapies to tissues that have been difficult to reach, such as the muscle and brain.

Therapies 52

Therapies Engineering Virus Packaging 52

DrugBank

JUNE 13, 2024

These microscopic particles, which are smaller than 100 nanometers, can be engineered to deliver drugs to specific targets with precision. Viral vectors, engineered from modified viruses that have lost their disease-causing potential, have emerged as a powerful tool for this purpose.

Therapies 98

Therapies Drugs DNA Treatment 98

The Pharma Data

OCTOBER 19, 2020

Spectrum’s self-contained saliva collection kit provides critical sample consistency while suspending and neutralizing viral RNA transcripts completely inactivating the live virus. “The SDNA-1000 was engineered to innovate molecular diagnostic applications using saliva,” said Jeremy Johnson, VP of Engineering at Spectrum Solutions.

RNA 52

RNA DNA Engineering Virus 52

The Pharma Data

APRIL 15, 2022

Designed computationally and refined in the laboratory, the new protein therapies thwarted infection by interfering with the virus’ ability to enter cells. The top protein neutralized the virus with similar or greater potency than antibody treatments with Emergency Use Authorization status from the U.S. 2 omicron subvariant.

Treatment 52

Treatment Virus Laboratories Engineering 52

Drug Target Review

SEPTEMBER 28, 2023

Because I was put into the highest tier, I was automatically mapped to the engineering track. For security reasons, at the time COVID-19 had to be grown in BSL-3 labs, which can be dangerous because they were handling the live virus. These are synthetic, not the live virus, so do not pose a risk of infection.

DNA 116

DNA Organic Chemistry Science Virus 116

Drug Target Review

SEPTEMBER 11, 2023

After numerous mutations of the StcE mucinase, the team developed a version known as eStcE (“engineered sticky”) that met their requirements. Their findings, published in Nature Biotechnology, have broad applications as mucins are associated with many diseases, including cystic fibrosis, respiratory diseases, and viruses.

Immune Response 116

Immune Response Research Engineering Virus 116

Drug Target Review

FEBRUARY 7, 2024

Third, since SARS-CoV-2 replicates faster than common cold coronaviruses, there are larger numbers of virus particles to fragment. Maybe the next pandemic respiratory virus will be an influenza virus rather than a coronavirus. Second, SARS-CoV-2 has many more of these high scoring fragments compared to common cold coronaviruses.

RNA 69

RNA Virus Immune Response Engineering 69

The Pharma Data

DECEMBER 31, 2020

Prior to joining Taysha, Gara served as vice president of Pharmaceutical Engineering at Sarepta, where he led and managed manufacturing operations for all gene therapy products. Before Sarepta, he served as vice president of Technical Operations and Engineering at AveXis, a Novartis company. Source link.

Engineering 52

Engineering Pharmaceuticals Clinical Development Virus 52

Broad Institute

MAY 30, 2023

She worked long shifts to help quickly deliver test results, while watching Broad engineers develop faster, more automated ways to process more COVID tests. In the summer of 2020, she joined the Broad Institute of MIT and Harvard as one of the first accessioning technicians in the COVID-19 diagnostic lab in the Broad’s Genomics Platform.

Research 89

Research Medical Schools Doctors Science 89

Drug Target Review

DECEMBER 5, 2024

2,3 Engineering both the capsid and DNA cargo improves the safety and efficacy of rAAV vectors and expands their clinical applications. Adeno-associated virus as a delivery vector for gene therapy of human diseases. Adeno-associated virus-based gene therapy for lifelong correction of genetic disease. Labcompare. 2024;9(1):78.

Therapies 52

Therapies Virus DNA Compliance 52

The Pharma Data

DECEMBER 23, 2020

. “A mask definitely helps, but if the people are very close to each other, there is still a chance of spreading or contracting the virus,” said study co-author Krishna Kota, an associate professor of mechanical and aerospace engineering. A single sneeze can carry up to 200 million tiny virus particles.

Virus 52

Virus Engineering Research 52

The Pharma Data

AUGUST 16, 2021

During challenge with the original SARS-CoV-2 virus, animals vaccinated with CV2CoV were found to be better protected based on highly effective clearance of the virus in the lungs and nasal passages. Clearance of the virus in the lungs and nasal passages of the animals was tested following challenge infection with the original virus.

Immune Response 52

Immune Response Vaccine Virus RNA 52

The Pharma Data

DECEMBER 10, 2020

In the study, immunization with the hAd5-COVID-19 vaccine inhibited SARS-CoV-2 virus replication in 100% (10 of 10) of Rhesus macaques, with a drop in viral replication starting on the first day of vaccine administration, and undetectable viral levels as early as three to five days post-challenge in most of the animals.

Vaccine 52

Vaccine Immune Response Virus Clinical Trials 52

The Pharma Data

APRIL 14, 2021

PREVEXXION ® RN is the results of unprecedented engineering design in vaccinology which brings optimal safety and efficacy balance. The product’s early Marek’s disease onset of immunity from five days of age provides full protection of broilers, layers and breeders against the most virulent Marek’s disease virus strains.

Vaccine 52

Vaccine Disease Virus Engineering 52

The Pharma Data

JANUARY 4, 2021

USA) to develop and commercialize gene therapy products based on adeno-associated virus (AAV) vectors to treat inherited eye diseases. The companies will use ViGeneron’s proprietary vgAAV, novel engineered AAV capsids, to efficiently transduce retinal cells via intravitreal injections. (Nasdaq: BIIB, Cambridge, Mass.,

Therapies 52

Therapies Virus Disease Licensing 52