Chemical Biology and Drug Design

NOVEMBER 20, 2023

Sorafenib and glimepiride simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells. Moreover, both compounds simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells.

Small Molecule 100

Small Molecule Protein Expression FDA Approval FDA 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Inflammatory factors in the serum were measured using ELISA assays, immunohistochemistry and qRT-PCR were performed to determine macrophage numbers and the expression of iNOS, CD86, Arg-1, and Mrc1 in myocardial tissue. The protein expression levels of TLR4, Myd88, and NF-κB in myocardial tissue were assessed through western blot analysis.

Biochemical Assays 100

Biochemical Assays Protein Expression Drugs Research 100

Drug Discovery World

JUNE 12, 2024

Our goal is to overcome this challenge to improve clinical decision-making in the first line setting and, ultimately, patient outcomes.”

Protein Expression 130

Protein Expression Medical Schools Treatment Therapies 130

Chemical Biology and Drug Design

DECEMBER 20, 2023

The protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), matrix metalloproteinase (MMP)-2, and MMP-9 were detected by western blotting. Besides, the decreased Nrf2 expression reduced the protein expression levels of MMP-2 and MMP-9 ( p  < .05).

Protein Expression 100

Protein Expression Treatment Drugs Research 100

Drug Discovery World

JUNE 14, 2023

While no changes to reporting terminology are recommended, the guideline update emphasizes awareness of the new clinical significance of the specific HER2 protein levels reported. It used the current CAP/ASCO testing algorithm for HER2 protein expression levels.

Protein Expression 130

Protein Expression Clinical Trials Trials Laboratories 130

Drug Discovery World

FEBRUARY 1, 2024

Kyverna’s CD19 CAR T-cell therapy, KYV-101, specifically targets CD19, a protein expressed on the surface of B cells, which is involved in various types of autoimmune diseases. “We were able to achieve a process minimisation without compromising the quality of the operations or the characteristics of the resulting product.”

Protein Expression 147

Protein Expression Therapies Production Disease 147

Drug Discovery World

JUNE 23, 2023

Elevidys addresses the root genetic cause of Duchenne – mutations in the dystrophin gene that result in the lack of dystrophin protein – by delivering a gene that codes for a shortened form of dystrophin to muscle cells known as Elevidys micro-dystrophin.

FDA Approval 243

FDA Approval Therapies FDA Protein Expression 243

Chemical Biology and Drug Design

JUNE 21, 2023

The results showed that hsa-miR-503-5p showed high expression, while its target gene CTDSPL presented decreased expression in LUAD. Hsa-miR-503-5p also had high expression in cisplatin-resistant LUAD cells. Binding relationship between the two genes was verified by dual-luciferase reporter assay.

Regulations 100

Regulations Protein Expression Bioinformatics Drugs 100

Chemical Biology and Drug Design

FEBRUARY 19, 2024

SIRT1 expression was enhanced by Icariin, and its knockdown suppressed Icariin-induced BMSC osteogenic differentiation. Moreover, deubiquitinating enzyme USP47 could stabilize SIRT1 protein expression.

Regulations 100

Regulations Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 5, 2024

The results showed that CA-COS inhibited nitric oxide (NO) production and downregulated the gene expression of nitric oxide synthase (iNOS), and cytokines such as tumor necrosis factor-alpha (TNF-α), IL-1β, and IL-6 without cytotoxic effect.

Protein Expression 100

Protein Expression Therapies Production Disease 100

Chemical Biology and Drug Design

FEBRUARY 15, 2024

RT-qPCR and western blotting were used to test the mRNA and protein expression levels of IL-17 and retinoid-related orphan receptor-γt (RORγt). PGE2 was highly expressed in the DE mouse model. The mRNA and protein levels of IL-17 and the key Th17 transcription factor RORγt were increased in tissues of the DE mice.

Disease 100

Disease Protein Expression Regulations Treatment 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

Moreover, mechanism research illustrates these natural compounds treat influenza A virus through key targets TLR4, TNF, and IL6 (high fever, acute respiratory distress syndrome), MAPK1, and MAPK3 (MAPK signaling pathway, viral RNP export, and viral protein expression), IL1B (NOD-like receptor signaling pathway, suppressed maturation of pro-IL-1β and (..)

Virus 100

Virus Protein Expression Vaccine Research 100

Drug Discovery Today

JANUARY 12, 2021

Exploratory research project aims to develop novel protein expression systems via cell reprogramming

Molecular Biology 113

Molecular Biology Laboratories Protein Expression Research 113

Drug Discovery World

MAY 30, 2024

In research, it allows us to directly study vaccine-responsive cells, for example their genomic composition and protein expression. Cells from responding lymph nodes are collected by means of ultrasound guided fine needle aspiration after immunisation in the arm. This is an established test in the clinic.

Vaccine 147

Vaccine Protein Expression Clinical Trials Immune Response 147

Chemical Biology and Drug Design

AUGUST 23, 2023

We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. The protein expressions were detected via western blot. Ligustrazine at doses of 100 and 1000 μg/mL was administrated in Kupffer cells isolated from THS rats.

Protein Expression 100

Protein Expression Protein Production Production Treatment 100

Chemical Biology and Drug Design

AUGUST 21, 2023

Further, the study detected the effect of SA on cell apoptosis, lipid peroxidation, Fe 2+ level, and ferroptosis-related proteins expression. Finally, the effect of HMGB1 expression on SA in H/R stimulation was studied.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

SEPTEMBER 21, 2023

Colonic pathological changes were analyzed by hematoxylin–eosin staining, and inflammatory factor expressions in serum were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and western blot were performed to quantify ferroptosis-related protein expressions.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 2, 2023

Linagliptin administered to the type 1 diabetic mouse heart significantly reduced the expression levels of the total and cleaved forms of ATF6, ATF4, and p-JNK, caspase 3. According to ELISA findings, TUDCA was more effective in reducing NOX 1 and MDA levels than linagliptin.

Protein Expression 100

Protein Expression Therapies Treatment Research 100

Drug Discovery World

SEPTEMBER 8, 2022

Pleco claims that its novel Plecoid therapies have the potential to positively change the balance of protein expression within the cancer microenvironment, removing the burden of toxic metals within the cell, thereby improving the effectiveness of existing chemotherapy.

Protein Expression 130

Protein Expression Drugs Government FDA 130

Chemical Biology and Drug Design

AUGUST 20, 2023

Hederagenin attenuated HG-induced increase in mRNA and protein expression of NLRP3, ASC, and IL-1β. The secretion levels of fibrosis-related biomarkers were analyzed by ELISA. Results showed that hederagenin reduced HG-induced proliferation increase in HRMCs and HRPTEpiCs. IV, PAI-1, and TGF-β1.

Bioinformatics 100

Bioinformatics Protein Expression Research 100

Chemical Biology and Drug Design

AUGUST 9, 2023

A label-free proteomics technology was employed to investigate alterations in protein expression in LoVo cells treated with plumbagin. Cell cycle analysis and cell apoptosis analysis were conducted to break down the anticancer impact of plumbagin on LoVo cells.

Bioinformatics 100

Bioinformatics Protein Expression Treatment Research 100

Drug Discovery World

AUGUST 9, 2022

The company also offers services for inhibition screening, protein expression and drug metabolite generation. Cypex’s product portfolio contains more than 115 recombinant enzymes, including CYPs, UGTs, CES, and AOX, and companion chemicals and media.

Protein Expression 130

Protein Expression Pharmaceutical Companies Production International 130

Chemical Biology and Drug Design

JUNE 8, 2023

In addition, SAS treatment caused a significant decrease in the mRNA and protein expression of xCT and GPX4, and a significant increase in ACSL4 expression in TE-1 cells treated with SAS. Flow cytometry results showed that the ferroptosis level was significantly increased after SAS treatment.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

DECEMBER 8, 2023

BoNT/A (1 nM) attenuated LPS/ATP-stimulated inhibition of viability and CAMP expression and upregulation of inflammatory mediators, pyroptosis-related proteins, and ELANE expression in rat DRG neurons, which was counteracted by CAMP silencing.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

APRIL 15, 2023

The mRNA and protein expression levels of p53, p-glycoprotein (P-gp), ATM, ATR, CHK1, and CHK2 were assessed through qRT-PCR and western blotting. OECM-1 and OECM-1/PTX were transfected with miR-34 mimic and inhibitor. Cellular proliferation and apoptosis were evaluated through MTT assay and flow cytometry, respectively.

DNA 100

DNA Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 4, 2024

The expression of miR-181a-3p was inhibited; however, SHQ1 expression was increased by β-sitosterol treatment of A549/anlotinib cells. The inhibition of SHQ1, ATF6, and GRP78 protein expression by β-sitosterol in A549/anlotinib cells was rescued by increased miR-181a-3p.

Protein Expression 100

Protein Expression Regulations Treatment Drugs 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

The mRNA or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real-time polymerase chain reaction or western blot.

Regulations 100

Regulations Protein Expression Treatment Research 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Elisa's result showed that CQQNC can significantly decrease the expression levels of cPLA2, sPLA2, PGE2, cAMP, and 15-PGDH after stimulating IL-1β to bEnd.3 3 cells in a dose-dependent manner ( p  < .01, 01, p  < .001, 001, p  < .0001). 3 ( p  < .0001, 0001, p  < .001, 001, p  < .01).

Treatment 100

Treatment Protein Expression Research 100

Chemical Biology and Drug Design

SEPTEMBER 25, 2023

Also, Vinp downregulated α-Syn protein expression and MDA level, while upregulated SOD activity in the striatum of PD rats. Vinp treatment increased the horizontal movement frequency and number of squares crossed, reduced the contact time, and rotation frequency in PD rats.

Disease 100

Disease Protein Expression Treatment Research 100

Covalent Modifiers

DECEMBER 19, 2023

We further introduce a comprehensive list of proteins involved in splicing and leverage both cysteine- and protein-directed activity-based protein profiling (ABPP) data with electrophilic scout fragments to demonstrate covalent ligandability for many classes of splicing factors and splicing regulators in primary human T cells.

Protein Expression 75

Protein Expression Regulations 75

Chemical Biology and Drug Design

DECEMBER 6, 2023

In the analysis of network pharmacology, 269 pharmacological targets of SP, 449 pharmacological targets of FC, and 2569 targets of HS-related diseases were screened from the databases.

Molecular Biology 100

Molecular Biology Protein Expression Treatment Regulations 100

Covalent Modifiers

JANUARY 17, 2024

We further introduce a comprehensive list of proteins involved in splicing and leverage both cysteine- and protein-directed activity-based protein profiling (ABPP) data with electrophilic scout fragments to demonstrate covalent ligandability for many classes of splicing factors and splicing regulators in primary human T cells.

Protein Expression 59

Protein Expression Regulations 59

ASPET

JUNE 27, 2024

In MDA-MB-231 cell xenografts, NOSH-ASA reduced tumor size markedly, which was associated with reduced proliferation (decreased PCNA expression), induction of apoptosis (increased TUNEL positive cells), and increased ROS, while NF-kB and FoxM1 that were high in untreated xenografts were significantly reduced.

Therapies 40

Therapies Protein Expression 40

Chemical Biology and Drug Design

DECEMBER 15, 2023

μM), significantly and dose-dependently induced apoptosis of SCC-12 and SK-MEL-28 cells, as evidenced by the suppression of Bcl-2 and upregulation of Bax, cleaved caspase-3, caspase-9, and PARP protein expression levels. The most active compounds 11 (A431: IC 50  = 5.0 μM, μM, SCC-12: IC 50  = 2.9 μM, μM, SKMEL-28: IC 50  = 4.9 μM,

Small Molecule 100

Small Molecule Protein Expression Laboratories Research 100

ASPET

OCTOBER 20, 2023

Western blotting was utilized for protein expression and epigenetic studies utilized chromatin immunoprecipitation methods. Furthermore, KET restored acetylated histone occupancy at the Bdnf promoter IV and induced BDNF protein expression in DFP rats. mg/kg s.c., At 6-m following DFP exposure, KET (10 mg/kg, i.p.)

Protein Expression 40

Protein Expression Clinical Trials Therapies FDA 40

Drug Discovery World

SEPTEMBER 13, 2023

Official comments “Our study shows a dominant prevalence of unnoticed SARS-CoV-2 infection in patients hospitalised in our psychiatric department during the first wave of the Covid-19 pandemic.

Protein Expression 130

Protein Expression Clinical Trials Hospitals Trials 130

ASPET

AUGUST 31, 2023

At the same time, CBG and EAA did not modify the expression/structure of proteins in relation to the non-irradiated control keratinocytes in the case of an unaccompanied use, or slightly modified the protein profile when used in a mixture.

Protein Expression 40

Protein Expression Treatment 40