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Humanization of SLCO2B1 in rats increases rCYP3A1 protein expression but not the metabolism of erlotinib to OSI-420 [Metabolism, Transport, and Pharmacogenetics]

ASPET

Western blot analysis of rat liver lysates and microsomes confirmed higher rCYP3A1 protein expression in untreated SLCO2B1 +/+ compared to Slco2b1 -/ - rats.

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Covalent Targeting of Splicing in T Cells

Covalent Modifiers

We further introduce a comprehensive list of proteins involved in splicing and leverage both cysteine- and protein-directed activity-based protein profiling (ABPP) data with electrophilic scout fragments to demonstrate covalent ligandability for many classes of splicing factors and splicing regulators in primary human T cells.

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Covalent Targeting of Splicing in T Cells

Covalent Modifiers

We further introduce a comprehensive list of proteins involved in splicing and leverage both cysteine- and protein-directed activity-based protein profiling (ABPP) data with electrophilic scout fragments to demonstrate covalent ligandability for many classes of splicing factors and splicing regulators in primary human T cells.

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Remimazolam attenuates inflammation in bronchopneumonia through the inhibition of NLRP3 activity by PDPK1 ubiquitination

Chemical Biology and Drug Design

Remimazolam induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression in lung tissue of mice model. In vitro model, Remimazolam also induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression. Remimazolam interlinked PDPK1 protein.

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Mogrify enters research collaboration with the MRC Laboratory of Molecular Biology

Drug Discovery Today

Exploratory research project aims to develop novel protein expression systems via cell reprogramming

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Targeting Hepatocellular Carcinoma: Schisandrin A Triggers Mitochondrial Disruption and Ferroptosis

Chemical Biology and Drug Design

Biochemical kits was utilized for checking the ATP, mitochondrial DNA, MDA, GSH, and Fe 2+ levels in the Huh7 cells, and western blot for measuring the ferroptosis and AMPK/mTOR related-protein expression levels.

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Diosgenin inhibits proliferation and migration of ovarian cancer cells and induce apoptosis via upregulation of PTEN

Chemical Biology and Drug Design

OVCAR-3 and SKOV-3 cells were treated with diosgenin, cellular viability was assessed by MTT assay and apoptosis was measured by ELISA and evaluated the protein expression levels of apoptotic markers through western blotting. The protein expression levels of main components of PI3K signaling were evaluated via western blotting.