Zobio

NOVEMBER 2, 2023

In an earlier blog article, we discussed the issue of protein aggregation, how to detect it and how we screen solution conditions to overcome it. Many of the other issues with ill-behaved proteins.

Protein Expression 40

Protein Expression Engineering Drugs 40

Chemical Biology and Drug Design

NOVEMBER 20, 2023

Sorafenib and glimepiride simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells. Moreover, both compounds simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells.

Small Molecule 100

Small Molecule Protein Expression FDA Approval FDA 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Inflammatory factors in the serum were measured using ELISA assays, immunohistochemistry and qRT-PCR were performed to determine macrophage numbers and the expression of iNOS, CD86, Arg-1, and Mrc1 in myocardial tissue. The protein expression levels of TLR4, Myd88, and NF-κB in myocardial tissue were assessed through western blot analysis.

Biochemical Assays 100

Biochemical Assays Protein Expression Drugs Research 100

Chemical Biology and Drug Design

JUNE 21, 2023

The results showed that hsa-miR-503-5p showed high expression, while its target gene CTDSPL presented decreased expression in LUAD. Hsa-miR-503-5p also had high expression in cisplatin-resistant LUAD cells. Binding relationship between the two genes was verified by dual-luciferase reporter assay.

Regulations 100

Regulations Protein Expression Bioinformatics Drugs 100

Chemical Biology and Drug Design

DECEMBER 20, 2023

The protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), matrix metalloproteinase (MMP)-2, and MMP-9 were detected by western blotting. Besides, the decreased Nrf2 expression reduced the protein expression levels of MMP-2 and MMP-9 ( p  < .05).

Protein Expression 100

Protein Expression Treatment Drugs Research 100

ASPET

OCTOBER 20, 2023

Western blotting was utilized for protein expression and epigenetic studies utilized chromatin immunoprecipitation methods. Furthermore, KET restored acetylated histone occupancy at the Bdnf promoter IV and induced BDNF protein expression in DFP rats. mg/kg s.c., At 6-m following DFP exposure, KET (10 mg/kg, i.p.)

Protein Expression 100

Protein Expression Clinical Trials Therapies FDA 100

ASPET

AUGUST 31, 2023

At the same time, CBG and EAA did not modify the expression/structure of proteins in relation to the non-irradiated control keratinocytes in the case of an unaccompanied use, or slightly modified the protein profile when used in a mixture.

Protein Expression 100

Protein Expression Treatment 100

Chemical Biology and Drug Design

FEBRUARY 19, 2024

SIRT1 expression was enhanced by Icariin, and its knockdown suppressed Icariin-induced BMSC osteogenic differentiation. Moreover, deubiquitinating enzyme USP47 could stabilize SIRT1 protein expression.

Regulations 100

Regulations Protein Expression Research 100

Chemical Biology and Drug Design

MARCH 5, 2024

The results showed that CA-COS inhibited nitric oxide (NO) production and downregulated the gene expression of nitric oxide synthase (iNOS), and cytokines such as tumor necrosis factor-alpha (TNF-α), IL-1β, and IL-6 without cytotoxic effect.

Protein Expression 100

Protein Expression Therapies Disease Production 100

Chemical Biology and Drug Design

SEPTEMBER 26, 2024

Western blot and molecular docking indicated that compound 22 may exert antioxidant activity by activating Nrf2 protein expression. This might be due to the introduction of 2, 5-difluorobenzene sulfonate group in PF, which helps in scavenging free radicals under oxidative stress.

Protein Expression 100

Protein Expression Pharmaceuticals Treatment Disease 100

Chemical Biology and Drug Design

FEBRUARY 15, 2024

RT-qPCR and western blotting were used to test the mRNA and protein expression levels of IL-17 and retinoid-related orphan receptor-γt (RORγt). PGE2 was highly expressed in the DE mouse model. The mRNA and protein levels of IL-17 and the key Th17 transcription factor RORγt were increased in tissues of the DE mice.

Disease 100

Disease Protein Expression Regulations Treatment 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

Moreover, mechanism research illustrates these natural compounds treat influenza A virus through key targets TLR4, TNF, and IL6 (high fever, acute respiratory distress syndrome), MAPK1, and MAPK3 (MAPK signaling pathway, viral RNP export, and viral protein expression), IL1B (NOD-like receptor signaling pathway, suppressed maturation of pro-IL-1β and (..)

Virus 100

Virus Protein Expression Vaccine Research 100

ASPET

JULY 19, 2024

Subsequent targeted gene expression profiling revealed that OKN-007 significantly downregulated the immunosuppressive and immunometabolic enzyme indolamine 2,3-dioxygenase 1 (IDO1) (-11.27-fold

Protein Expression 100

Protein Expression Clinical Trials Treatment Regulations 100

Chemical Biology and Drug Design

AUGUST 23, 2023

We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. The protein expressions were detected via western blot. Ligustrazine at doses of 100 and 1000 μg/mL was administrated in Kupffer cells isolated from THS rats.

Protein Expression 100

Protein Expression Protein Production Treatment Production 100

Chemical Biology and Drug Design

AUGUST 21, 2023

Further, the study detected the effect of SA on cell apoptosis, lipid peroxidation, Fe 2+ level, and ferroptosis-related proteins expression. Finally, the effect of HMGB1 expression on SA in H/R stimulation was studied.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 2, 2023

Linagliptin administered to the type 1 diabetic mouse heart significantly reduced the expression levels of the total and cleaved forms of ATF6, ATF4, and p-JNK, caspase 3. According to ELISA findings, TUDCA was more effective in reducing NOX 1 and MDA levels than linagliptin.

Protein Expression 100

Protein Expression Therapies Treatment Research 100

Chemical Biology and Drug Design

APRIL 15, 2023

The mRNA and protein expression levels of p53, p-glycoprotein (P-gp), ATM, ATR, CHK1, and CHK2 were assessed through qRT-PCR and western blotting. OECM-1 and OECM-1/PTX were transfected with miR-34 mimic and inhibitor. Cellular proliferation and apoptosis were evaluated through MTT assay and flow cytometry, respectively.

DNA 100

DNA Protein Expression Research 100

Chemical Biology and Drug Design

SEPTEMBER 21, 2023

Colonic pathological changes were analyzed by hematoxylin–eosin staining, and inflammatory factor expressions in serum were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and western blot were performed to quantify ferroptosis-related protein expressions.

Protein Expression 100

Protein Expression Treatment Research 100

Chemical Biology and Drug Design

AUGUST 20, 2023

Hederagenin attenuated HG-induced increase in mRNA and protein expression of NLRP3, ASC, and IL-1β. The secretion levels of fibrosis-related biomarkers were analyzed by ELISA. Results showed that hederagenin reduced HG-induced proliferation increase in HRMCs and HRPTEpiCs. IV, PAI-1, and TGF-β1.

Bioinformatics 100

Bioinformatics Protein Expression Research 100

Chemical Biology and Drug Design

JUNE 8, 2023

In addition, SAS treatment caused a significant decrease in the mRNA and protein expression of xCT and GPX4, and a significant increase in ACSL4 expression in TE-1 cells treated with SAS. Flow cytometry results showed that the ferroptosis level was significantly increased after SAS treatment.

Protein Expression 100

Protein Expression Treatment Research 100

ASPET

JUNE 27, 2024

In MDA-MB-231 cell xenografts, NOSH-ASA reduced tumor size markedly, which was associated with reduced proliferation (decreased PCNA expression), induction of apoptosis (increased TUNEL positive cells), and increased ROS, while NF-kB and FoxM1 that were high in untreated xenografts were significantly reduced.

Therapies 100

Therapies Protein Expression 100

Chemical Biology and Drug Design

AUGUST 9, 2023

A label-free proteomics technology was employed to investigate alterations in protein expression in LoVo cells treated with plumbagin. Cell cycle analysis and cell apoptosis analysis were conducted to break down the anticancer impact of plumbagin on LoVo cells.

Bioinformatics 100

Bioinformatics Protein Expression Treatment Research 100

Chemical Biology and Drug Design

JANUARY 23, 2024

Elisa's result showed that CQQNC can significantly decrease the expression levels of cPLA2, sPLA2, PGE2, cAMP, and 15-PGDH after stimulating IL-1β to bEnd.3 3 cells in a dose-dependent manner ( p  < .01, 01, p  < .001, 001, p  < .0001). 3 ( p  < .0001, 0001, p  < .001, 001, p  < .01).

Treatment 100

Treatment Protein Expression Research 100

Chemical Biology and Drug Design

SEPTEMBER 25, 2023

Also, Vinp downregulated α-Syn protein expression and MDA level, while upregulated SOD activity in the striatum of PD rats. Vinp treatment increased the horizontal movement frequency and number of squares crossed, reduced the contact time, and rotation frequency in PD rats.

Disease 100

Disease Protein Expression Treatment Research 100

Chemical Biology and Drug Design

SEPTEMBER 24, 2023

The mRNA or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real-time polymerase chain reaction or western blot.

Regulations 100

Regulations Protein Expression Treatment Research 100

Chemical Biology and Drug Design

MARCH 4, 2024

The expression of miR-181a-3p was inhibited; however, SHQ1 expression was increased by β-sitosterol treatment of A549/anlotinib cells. The inhibition of SHQ1, ATF6, and GRP78 protein expression by β-sitosterol in A549/anlotinib cells was rescued by increased miR-181a-3p.

Protein Expression 100

Protein Expression Regulations Treatment Drugs 100

Chemical Biology and Drug Design

DECEMBER 8, 2023

BoNT/A (1 nM) attenuated LPS/ATP-stimulated inhibition of viability and CAMP expression and upregulation of inflammatory mediators, pyroptosis-related proteins, and ELANE expression in rat DRG neurons, which was counteracted by CAMP silencing.

Protein Expression 100

Protein Expression Treatment Research 100

Covalent Modifiers

DECEMBER 19, 2023

We further introduce a comprehensive list of proteins involved in splicing and leverage both cysteine- and protein-directed activity-based protein profiling (ABPP) data with electrophilic scout fragments to demonstrate covalent ligandability for many classes of splicing factors and splicing regulators in primary human T cells.

Protein Expression 81

Protein Expression Regulations 81

Drug Target Review

APRIL 5, 2024

Together, these attributes provide a strong foundation for protein expression with enough adaptability to produce much of the commercial and therapeutic protein market.

Protein Production 96

Protein Production Protein Expression Bioinformatics Production 96

Chemical Biology and Drug Design

DECEMBER 6, 2023

In the analysis of network pharmacology, 269 pharmacological targets of SP, 449 pharmacological targets of FC, and 2569 targets of HS-related diseases were screened from the databases.

Molecular Biology 100

Molecular Biology Protein Expression Treatment Regulations 100

Chemical Biology and Drug Design

DECEMBER 15, 2023

μM), significantly and dose-dependently induced apoptosis of SCC-12 and SK-MEL-28 cells, as evidenced by the suppression of Bcl-2 and upregulation of Bax, cleaved caspase-3, caspase-9, and PARP protein expression levels. The most active compounds 11 (A431: IC 50  = 5.0 μM, μM, SCC-12: IC 50  = 2.9 μM, μM, SKMEL-28: IC 50  = 4.9 μM,

Small Molecule 100

Small Molecule Protein Expression Laboratories Research 100

Covalent Modifiers

JANUARY 17, 2024

We further introduce a comprehensive list of proteins involved in splicing and leverage both cysteine- and protein-directed activity-based protein profiling (ABPP) data with electrophilic scout fragments to demonstrate covalent ligandability for many classes of splicing factors and splicing regulators in primary human T cells.

Protein Expression 63

Protein Expression Regulations 63

Fierce BioTech

SEPTEMBER 11, 2024

Comparable Results: Achieve cell growth and protein expression levels similar to those obtained with smaller-scale shake flasks. Enhanced Cellular Environment: Learn about the unique design of our Nalgene flask that minimizes damaging shear stress, fostering a gentle environment for mammalian cells. Register now to secure your spot!

Protein Expression 52

Protein Expression Production 52

The Pharma Data

JANUARY 16, 2021

Etta Biotech”), to set up a high titer transient protein expression platform for high quality protein production using JS Bio’s transient transfection media. JS Bio becomes the exclusive cell culture supplier for Etta Biotech’s transient transfection high titer protein expression platform.

Protein Expression 52

Protein Expression Protein Production Production Drug Development 52

Drug Target Review

JANUARY 26, 2024

To test whether the mouse-made human ACE2 proteins were functional, the researchers used numerous methods, which included demonstrating that a psuedovirus containing the Covid spike protein could bind to the receptors and infect cells.

Production 114

Production DNA Virus Protein Expression 114

Codon

DECEMBER 10, 2023

Predictive models for biology will span all scales, from individual proteins to the culture conditions in which cells grow. Large-scale experiments suggest that <50% of bacterial proteins and <15% of non-bacterial proteins express within E. coli , yeast, and other types of organisms.

Protein Expression 87

Protein Expression DNA Laboratories Engineering 87

Drug Target Review

OCTOBER 6, 2023

Focusing on SRC-3 within the nucleus activates a series of changes in protein expression that have a profound impact of enhancing the immune system’s ability to engage and attack cancer cells. By working directly at this level in the Treg, CoRegen can precisely manipulate its cellular activity at its source, the TME.

Small Molecule 111

Small Molecule Engineering Protein Expression Therapies 111