Mogrify enters research collaboration with the MRC Laboratory of Molecular Biology
Drug Discovery Today
JANUARY 12, 2021
Exploratory research project aims to develop novel protein expression systems via cell reprogramming
Targeting Hepatocellular Carcinoma: Schisandrin A Triggers Mitochondrial Disruption and Ferroptosis
Chemical Biology and Drug Design
DECEMBER 12, 2024
ABSTRACT The main focus of this research was to examine SchA's role in the hepatocellular carcinoma (HCC) development. Biochemical kits was utilized for checking the ATP, mitochondrial DNA, MDA, GSH, and Fe 2+ levels in the Huh7 cells, and western blot for measuring the ferroptosis and AMPK/mTOR related-protein expression levels.
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Remimazolam attenuates inflammation in bronchopneumonia through the inhibition of NLRP3 activity by PDPK1 ubiquitination
Chemical Biology and Drug Design
JANUARY 5, 2024
Remimazolam induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression in lung tissue of mice model. In vitro model, Remimazolam also induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression. Remimazolam interlinked PDPK1 protein.
Scientific workflow for hypothesis testing in drug discovery: Part 2 of 3
Drug Target Review
JANUARY 14, 2025
Operating a workflow, such as the one described in Figure 1, involves several key considerations that affect both the accuracy of results and the efficiency of the research process. Defining the research question A well-defined research question is the cornerstone of an effective scientific workflow in drug discovery.
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Artesunate Suppresses the Migration and Invasion of Thyroid Cancer Cells via Upregulating PTEN to Block M2 Polarization of Tumor‐Associated Macrophages
Chemical Biology and Drug Design
MARCH 18, 2025
The mRNA or protein expressions of examined genes were measured by quantitative real-time polymerase chain reaction or Western blot. The viability, migration, and invasion of TC cells were detected by cell counting kit-8, wound healing, and transwell assays.
Matrine exerts an anti?tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo
Chemical Biology and Drug Design
SEPTEMBER 6, 2023
Western blot analysis was applied to detect the protein expression of apoptosis HN1. The effects of matrine on tumor growth, protein expression of HN1, and apoptosis in vivo were validated by xenograft tumor models and histology. HN1 overexpression perceptibly reversed the above-mentioned additive effect in vitro.
Discovery of small molecule c?Maf inhibitors using molecular docking?based virtual screening, molecular dynamics simulation, and biological evaluation
Chemical Biology and Drug Design
NOVEMBER 20, 2023
Sorafenib and glimepiride simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells. Moreover, both compounds simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells.
Small Molecule 100
100 
Ketamine produces antidepressant effects by inhibiting histone deacetylases and upregulating hippocampal BDNF levels in a DFP-based rat model of Gulf War Illness [Toxicology]
ASPET
OCTOBER 20, 2023
Western blotting was utilized for protein expression and epigenetic studies utilized chromatin immunoprecipitation methods. Furthermore, KET restored acetylated histone occupancy at the Bdnf promoter IV and induced BDNF protein expression in DFP rats. mg/kg s.c., At 6-m following DFP exposure, KET (10 mg/kg, i.p.)
Diosgenin inhibits proliferation and migration of ovarian cancer cells and induce apoptosis via upregulation of PTEN
Chemical Biology and Drug Design
MARCH 27, 2024
OVCAR-3 and SKOV-3 cells were treated with diosgenin, cellular viability was assessed by MTT assay and apoptosis was measured by ELISA and evaluated the protein expression levels of apoptotic markers through western blotting. The protein expression levels of main components of PI3K signaling were evaluated via western blotting.
Curcumin activates the JNK signaling pathway to promote ferroptosis in colon cancer cells
Chemical Biology and Drug Design
MARCH 5, 2024
Curcumin modulates the gene and protein expression levels of ferroptosis mediators via JNK signaling. In addition, curcumin modulated the mRNA and protein expression levels of ferroptosis-related proteins including ACSL4, GPx4 and FTH1 and suppression of JNK signaling.
Discovery of natural multi?targets neuraminidase inhibitor glycosides compounds against influenza A virus through network pharmacology, virtual screening, molecular dynamics simulation, and in vitro experiment
Chemical Biology and Drug Design
SEPTEMBER 24, 2023
Therefore, based on ADMET properties, eight optimal natural multi-targets NIs glycosides compounds (IC 50 = 0.094–97.275 μM) are found from radix glycyrrhizae, flos sophorae, caulis spatholobi, radix astragali, radix glycyrrhizae, semen astragali complanati , and common fenugreek seed through network pharmacology, molecular docking, dynamics simulation, (..)
Virus 100
100 
Hsa?miR?503?5p regulates CTDSPL to accelerate cisplatin resistance and angiogenesis of lung adenocarcinoma cells
Chemical Biology and Drug Design
JUNE 21, 2023
The results showed that hsa-miR-503-5p showed high expression, while its target gene CTDSPL presented decreased expression in LUAD. Hsa-miR-503-5p also had high expression in cisplatin-resistant LUAD cells. Binding relationship between the two genes was verified by dual-luciferase reporter assay.
Regulations 100
100 
Betulinic acid reduces inflammation in rats with sepsis?induced myocardial dysfunction by inhibiting TLR4/MyD88/NF??B signaling pathway
Chemical Biology and Drug Design
JANUARY 23, 2024
Inflammatory factors in the serum were measured using ELISA assays, immunohistochemistry and qRT-PCR were performed to determine macrophage numbers and the expression of iNOS, CD86, Arg-1, and Mrc1 in myocardial tissue. The protein expression levels of TLR4, Myd88, and NF-κB in myocardial tissue were assessed through western blot analysis.
Triptolide inhibits proliferation and invasion of colorectal cancer cells by blocking Nrf2 expression
Chemical Biology and Drug Design
DECEMBER 20, 2023
The protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), matrix metalloproteinase (MMP)-2, and MMP-9 were detected by western blotting. Besides, the decreased Nrf2 expression reduced the protein expression levels of MMP-2 and MMP-9 ( p < .05).
Jasminoidin reduces ischemic stroke injury by regulating microglia polarization via PASK?EEF1A1 axis
Chemical Biology and Drug Design
SEPTEMBER 24, 2023
This research is powered to probe whether the molecular mechanism of JAS for IS treatment is coupled with microglia polarization. The mRNA or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real-time polymerase chain reaction or western blot.
Regulations 100
100 Synthesis, Antioxidant Activity, and Molecular Docking of Novel Paeoniflorin Derivatives
Chemical Biology and Drug Design
SEPTEMBER 26, 2024
Western blot and molecular docking indicated that compound 22 may exert antioxidant activity by activating Nrf2 protein expression. This might be due to the introduction of 2, 5-difluorobenzene sulfonate group in PF, which helps in scavenging free radicals under oxidative stress.
Icariin promotes osteogenic differentiation of human bone marrow mesenchymal stem cells by regulating USP47/SIRT1/Wnt/??catenin
Chemical Biology and Drug Design
FEBRUARY 19, 2024
SIRT1 expression was enhanced by Icariin, and its knockdown suppressed Icariin-induced BMSC osteogenic differentiation. Moreover, deubiquitinating enzyme USP47 could stabilize SIRT1 protein expression.
Regulations 100
100 
Caffeic acid?grafted chitooligosaccharides downregulate MAPK and NF?kB in RAW264.7 cells
Chemical Biology and Drug Design
MARCH 5, 2024
The results showed that CA-COS inhibited nitric oxide (NO) production and downregulated the gene expression of nitric oxide synthase (iNOS), and cytokines such as tumor necrosis factor-alpha (TNF-α), IL-1β, and IL-6 without cytotoxic effect.
The mechanism of Semen Persicae?Flos Carthami in treating hypertrophic scar: A study based on network pharmacological analysis and in vitro experiments
Chemical Biology and Drug Design
DECEMBER 6, 2023
To sum up, guided by network pharmacological analysis and previous literature research, a p53 interfering cell model has been established with the help of shRNA interference technology to investigate the anti-HS effect of SP-FC dependent on p53 in this study.
Ligustrazine improves the compensative effect of Akt survival signaling to protect liver Kupffer cells in trauma?hemorrhagic shock rats
Chemical Biology and Drug Design
AUGUST 23, 2023
We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. The protein expressions were detected via western blot. Ligustrazine at doses of 100 and 1000 μg/mL was administrated in Kupffer cells isolated from THS rats.
Syringic acid suppresses ferroptosis of skeletal muscle cells to alleviate lower limb ischemia/reperfusion injury in mice via the HMGB1 pathway
Chemical Biology and Drug Design
AUGUST 21, 2023
Further, the study detected the effect of SA on cell apoptosis, lipid peroxidation, Fe 2+ level, and ferroptosis-related proteins expression. Finally, the effect of HMGB1 expression on SA in H/R stimulation was studied.
Evaluation of linagliptin and insulin combined therapy on unfolded protein response in type 1 diabetic mouse heart
Chemical Biology and Drug Design
AUGUST 2, 2023
Linagliptin administered to the type 1 diabetic mouse heart significantly reduced the expression levels of the total and cleaved forms of ATF6, ATF4, and p-JNK, caspase 3. According to ELISA findings, TUDCA was more effective in reducing NOX 1 and MDA levels than linagliptin.
MiR?34 by targeting p53 induces apoptosis and DNA damage in paclitaxel?resistant human oral squamous carcinoma cells
Chemical Biology and Drug Design
APRIL 15, 2023
The mRNA and protein expression levels of p53, p-glycoprotein (P-gp), ATM, ATR, CHK1, and CHK2 were assessed through qRT-PCR and western blotting. OECM-1 and OECM-1/PTX were transfected with miR-34 mimic and inhibitor. Cellular proliferation and apoptosis were evaluated through MTT assay and flow cytometry, respectively.
DNA 100
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A therapy candidate for fatal prion diseases turns off disease-causing gene
Broad Institute
JUNE 27, 2024
By Greta Friar, Whitehead Institute June 27, 2024 Images of a mouse brain show the effect of a technology called CHARM in turning off the expression of a gene in the brain. Previous research has shown that as little as 21 percent elimination of the protein can improve symptoms. Credit: Neumann EN, Bertozzi TM, et al.
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142 Prostaglandin E2 may clinically alleviate dry eye disease by inducing Th17 cell differentiation
Chemical Biology and Drug Design
FEBRUARY 15, 2024
RT-qPCR and western blotting were used to test the mRNA and protein expression levels of IL-17 and retinoid-related orphan receptor-γt (RORγt). PGE2 was highly expressed in the DE mouse model. The mRNA and protein levels of IL-17 and the key Th17 transcription factor RORγt were increased in tissues of the DE mice.
Disease 100
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Baicalein, a component of banxia xiexin decoction, alleviates CPT?11?induced gastrointestinal dysfunction by inhibiting ALOX15?mediated ferroptosis
Chemical Biology and Drug Design
SEPTEMBER 21, 2023
Colonic pathological changes were analyzed by hematoxylin–eosin staining, and inflammatory factor expressions in serum were determined by enzyme-linked immunosorbent assay. Immunohistochemistry and western blot were performed to quantify ferroptosis-related protein expressions.
Hederagenin inhibits high glucose?induced fibrosis in human renal cells by suppression of NLRP3 inflammasome activation through reducing cathepsin B expression
Chemical Biology and Drug Design
AUGUST 20, 2023
Hederagenin attenuated HG-induced increase in mRNA and protein expression of NLRP3, ASC, and IL-1β. The secretion levels of fibrosis-related biomarkers were analyzed by ELISA. Results showed that hederagenin reduced HG-induced proliferation increase in HRMCs and HRPTEpiCs. IV, PAI-1, and TGF-β1.
Bioinformatics 100
100 Sulfasalazine inhibits esophageal cancer cell proliferation by mediating ferroptosis
Chemical Biology and Drug Design
JUNE 8, 2023
In addition, SAS treatment caused a significant decrease in the mRNA and protein expression of xCT and GPX4, and a significant increase in ACSL4 expression in TE-1 cells treated with SAS. Flow cytometry results showed that the ferroptosis level was significantly increased after SAS treatment.
Plumbagin induces apoptosis, cell cycle arrest, and inhibits protein synthesis in LoVo colon cancer cells: A proteomic analysis
Chemical Biology and Drug Design
AUGUST 9, 2023
A label-free proteomics technology was employed to investigate alterations in protein expression in LoVo cells treated with plumbagin. Cell cycle analysis and cell apoptosis analysis were conducted to break down the anticancer impact of plumbagin on LoVo cells.
Bioinformatics 100
100 
Vinpocetine improves dyskinesia in Parkinson's disease rats by reducing oxidative stress and activating the Wnt/??catenin signaling pathway
Chemical Biology and Drug Design
SEPTEMBER 25, 2023
Also, Vinp downregulated α-Syn protein expression and MDA level, while upregulated SOD activity in the striatum of PD rats. Vinp treatment increased the horizontal movement frequency and number of squares crossed, reduced the contact time, and rotation frequency in PD rats.
Disease 100
100 
What Biology Can Learn from Physics
Codon
DECEMBER 10, 2023
As money poured into wartime research programs, physics shifted from a field of brilliant individuals to one of well-managed teams. Biology has had a few large-scale research programs, such as the Human Genome Project , but nowhere near the same number as physics. For one, biology research is inherently broad.
Botulinum toxin type A ameliorates rat dorsal root ganglia neuron pyroptosis in postherpetic neuralgia by upregulating cathelicidin antimicrobial peptide to inhibit neutrophil elastase
Chemical Biology and Drug Design
DECEMBER 8, 2023
BoNT/A (1 nM) attenuated LPS/ATP-stimulated inhibition of viability and CAMP expression and upregulation of inflammatory mediators, pyroptosis-related proteins, and ELANE expression in rat DRG neurons, which was counteracted by CAMP silencing.
Investigation on the antipyretic mechanism of Chaiqin Qingning capsule for the treatment of fever based on network pharmacology, molecular docking, and in vitro experimental validation
Chemical Biology and Drug Design
JANUARY 23, 2024
Elisa's result showed that CQQNC can significantly decrease the expression levels of cPLA2, sPLA2, PGE2, cAMP, and 15-PGDH after stimulating IL-1β to bEnd.3 3 cells in a dose-dependent manner ( p < .01, 01, p < .001, 001, p < .0001). 3 ( p < .0001, 0001, p < .001, 001, p < .01).
Treatment 100
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SARS-CoV-2: advancing the production of the ACE2 protein
Drug Target Review
JANUARY 26, 2024
Researchers from Columbia University and the US Department of Energy (DOE)’s Brookhaven National Laboratory have elucidated a method to produce large quantities of the receptor that the SARS-CoV-2 spike protein uses to bind to the surface of human cells.
Production 114
114 ??Sitosterol attenuates anlotinib resistance in non?small cell lung cancer cells by inhibiting miR?181a?3p/SHQ1 signaling
Chemical Biology and Drug Design
MARCH 4, 2024
The expression of miR-181a-3p was inhibited; however, SHQ1 expression was increased by β-sitosterol treatment of A549/anlotinib cells. The inhibition of SHQ1, ATF6, and GRP78 protein expression by β-sitosterol in A549/anlotinib cells was rescued by increased miR-181a-3p.
Multifaceted approach toward mapping out the anticancer properties of small molecules via in vitro evaluation on melanoma and nonmelanoma skin cancer cells, and in silico target fishing
Chemical Biology and Drug Design
DECEMBER 15, 2023
μM), significantly and dose-dependently induced apoptosis of SCC-12 and SK-MEL-28 cells, as evidenced by the suppression of Bcl-2 and upregulation of Bax, cleaved caspase-3, caspase-9, and PARP protein expression levels. The most active compounds 11 (A431: IC 50 = 5.0 μM, μM, SCC-12: IC 50 = 2.9 μM, μM, SKMEL-28: IC 50 = 4.9 μM,
Small Molecule 100
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Women in Stem with Dr Beate Mueller-Tiemann
Drug Target Review
FEBRUARY 19, 2024
I studied and conducted research in Germany, France, Switzerland and the US. Throughout my career, I have had the privilege to apply what I learned from my research to help advance the life sciences industry. I spent many years researching and identifying new molecules with pharmacological activity.
SRC-3: CoRegen’s revolutionary approach to cancer
Drug Target Review
OCTOBER 6, 2023
Preclinical research demonstrated that removing SRC-3 from Tregs resulted in long-lasting tumour eradication and prevented growth of new tumours. Focusing on SRC-3 within the nucleus activates a series of changes in protein expression that have a profound impact of enhancing the immune system’s ability to engage and attack cancer cells.
Small Molecule 111
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What Biology Can Learn from Physics
Codon
DECEMBER 10, 2023
As money poured into wartime research programs, physics shifted from a field of brilliant individuals to one of well-managed teams. Biology has had a few large-scale research programs, such as the Human Genome Project , but nowhere near the same number as physics. For one, biology research is inherently broad.
The Future of Drug Discovery: Tackling the Undruggable with New Biotechnologies
DrugBank
JULY 24, 2024
This drug, which targets a specific protein-protein interaction (PPI), took over two decades of research and development but has proven highly effective in treating certain cancers. By blocking this interaction, researchers aim to unleash p53's full tumor-suppressing potential.
Gilead and Dragonfly Announce Strategic Research Collaboration to Develop Natural Killer Cell Engagers in Oncology and Inflammation
The Pharma Data
MAY 2, 2022
The target of DF7001 is 5T4, a protein expressed on cancer cells and stromal cells that support tumor growth associated with poor prognosis in several cancers, including non-small cell lung cancer (NSCLC), pancreatic cancer, breast cancer, and head and neck squamous cell carcinomas (HNSCC). View the full release here: [link].
Licensing 52
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Addressing increasingly resistant drugs by infectious agents
Drug Target Review
SEPTEMBER 4, 2023
Infectious agents’ resistance was described at the European Union Research Parliament “as a widespread, multifaceted phenomenon that affects both living things and the environment, as well as their capacity to survive in the presence of drugs intended to either kill or inactivate them.” References: Antunes Luisa (2023).
Drugs 111
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Driving efficiency across upstream bioprocess workflow
Drug Target Review
MARCH 15, 2024
As discussed above, recent advances in gene editing tools and transposon-based vectors have facilitated targeted and consistent cell line engineering for the development of protein expressing CHO cell lines. Disclaimer: For research use only. Anis works within the strategy group in Life Sciences at Revvity, Inc.
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Marina Saffold
Broad Institute
SEPTEMBER 26, 2024
Yes, it changed me as a researcher: I gained the skills to overcome obstacles in science, plan and execute my experiments, and dive deep into the background of a project to build confidence in my research area. Battaglia, Stanley Center for Psychiatric Research BSRP is nothing short of transformative.
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