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Listicle: Five tips on scaling up IVT for mRNA production

Drug Discovery World

The success of messenger RNA (mRNA) vaccines in combatting the global SARS-CoV-2 crisis brings a new era of vaccine development. IVT is a complex reaction, requiring a mixture of RNA polymerase and supplementary components (e.g., Also keep in mind that optimisation is not linear and can be process- and product-dependent.